Anti-Secretogranin II Antibody (26107)

$516.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,IHC Human, Rat, Squirrel Monkey
SKU: 26107 Categories: , ,
Overview
Product Name Anti-Secretogranin II Antibody (26107)
Description Anti-Secretogranin II Rabbit Polyclonal Antibody
Target Secretogranin II
Species Reactivity Human, Rat, Squirrel Monkey
Applications WB,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide derived from the N-terminus of mature SgII protein: QRNQLLGKEPDLRLENVQK (Accession no. AAA36607).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7 .4
Buffer Formulation Phosphate Buffered Saline
Buffer pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, rat, and squirrel monkey SgII.
Target Name Secretogranin-2
Target ID Secretogranin II
Uniprot ID P13521
Alternative Names Chromogranin-C, Secretogranin II, SgII [Cleaved into: Secretoneurin
Gene Name SCG2
Sequence Location Secreted. Note=Neuroendocrine and endocrine secretory granules.
Biological Function Neuroendocrine protein of the granin family that regulates the biogenesis of secretory granules. {PubMed:19357184}.
Research Areas Hormones/Proteins
Background Secretogranin II (SgII), encoded by the gene SCG2, is a member of the granin family of neuroendocrine secretory proteins. The granin family also includes chromogranin A (CgA), chromogranin B (CgB), and several other secretogranin-like proteins. Studies in rodents suggest that the full-length protein, SgII, is involved in the packaging or sorting of peptide hormones and neuropeptides into secretory vesicles. SgII occurs in a variety of neoplasms arising from endocrine and neuroendocrine cells and can be a useful histological tumor marker for those cells.
Application Images
Description Immunoblotting: use at 0.5-1.0ug/ml. PC12 extract (1ug) Although the predicted mw of SgII is ~71kDa, the highly acidic nature of SgII causes it to run at a higher mw in SDS-PAGE.
Description Immunohistochemistry: use at 2-5ug/ml. Squirrel monkey pituitary gland, staining visualized with Texas Red-labeled goat anti-rabbit antibody.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C to -70°C. Avoid repeated freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-1.0ug/mL.

Immunohistochemistry: use at 2-5ug/mL.
References & Data Sheet
References Vasauskas AA et al. 2011. Regulation and distribution of squirrel monkey chorionic gonadotropin and secretogranin II in the pituitary. General and Comparative Endocrinology 170: 509-513.