Anti-Retinoblastoma protein phosphoserine 249/phosphothreonine 252 Antibody (3112)

$200.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
Rabbit	25ug	WB	Human

SKU: 3112 Categories: Antibody Products, Cancer Research Antibodies, Products, Retinoblastoma Protein Antibodies

Overview

Product Name Anti-Retinoblastoma protein phosphoserine 249/phosphothreonine 252 Antibody (3112)

Description Anti-Retinoblastoma phosphoserine 249/phosphothreonine 252 Rabbit Polyclonal Antibody

Target Retinoblastoma protein phosphoserine 249/phosphothreonine 252

Species Reactivity Human

Applications WB

Host Rabbit

Clonality Polyclonal

Immunogen Phosphopeptide region of human Rb protein containing Serine 249 and threonine 252.

Properties

Form Liquid

Concentration 1.0 mg/mL

Formulation PBS, pH 7.4, 0.05% sodium azide, 1mg/ml BSA.

Buffer Formulation Phosphate Buffered Saline

Buffer pH pH 7.4

Buffer Anti-Microbial 0.05% Sodium Azide

Buffer Protein Stabilizer 1 mg/mL Bovine Serum Albumin

Format Purified

Purification Purified by immunoaffinity chromatography

Specificity This antibody recognizes human Rb protein phosphorylated at serine 249 and threonine 252. Reactivity with other species has not been investigated.

Target Name Retinoblastoma-associated protein

Target ID Retinoblastoma protein phosphoserine 249/phosphothreonine 252

Uniprot ID P06400

Alternative Names p105-Rb, p110-RB1, pRb, Rb, pp110

Gene Name RB1

Gene ID 5925

Accession Number NP_000312

Post Translational Modification Retinoblastoma protein phosphoserine 249/phosphothreonine 252

Target Phospho-tyr252

Sequence Location Nucleus

Biological Function Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). {UniProtKB:P13405, UniProtKB:P33568, PubMed:10499802, PubMed:15084261}.; (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. {PubMed:1316611, PubMed:17974914, PubMed:18701596, PubMed:2839300, PubMed:8892909}.

Research Areas Cancer Research

Handling

Storage Store at -80°C. Prepare appropriate aliquots to avoid multiple freeze-thaw cycles.

Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.

Application Instructions Immunoblotting: use at 0.25-1ug/ml.

End users should determine optimal concentration for their application.

Data Sheet