Anti-Replication Factor C (p37) Antibody (56253)

$503.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF Human, Yeast
SKU: 56253 Categories: , ,
Overview
Product Name Anti-Replication Factor C (p37) Antibody (56253)
Description Anti-Replication Factor C p37 Mouse Monoclonal Antibody
Target Replication Factor C (p37)
Species Reactivity Human, Yeast
Applications WB,ICC/IF
Host Mouse
Clonality Monoclonal
Clone ID 1320
Isotype IgG2b
Immunogen Recombinant protein corresponding to the p37 subunit of human Replication Factor C expressed in E. coli.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes the p37 subunit of human and yeast (S. cerevisiae) Replication Factor C (RFC). RFC is a heteropentameric protein consisting of p140, p40, p38, p37, and p36 subunits, RFC participates in targeting DNA polymerase delta processivity factor (PCNA) on to a primed DNA template during DNA synthesis and repair. RFC has also been associated with DNA repair in conjunction with a PCNA-like heterotrimer of Hus1, Rad1, and Rad9.
Target Name Replication factor C subunit 2
Target ID Replication Factor C (p37)
Uniprot ID P35250
Alternative Names Activator 1 40 kDa subunit, A1 40 kDa subunit, Activator 1 subunit 2, Replication factor C 40 kDa subunit, RF-C 40 kDa subunit, RFC40
Gene Name RFC2
Sequence Location Nucleus
Biological Function The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP (By similarity). {ECO:0000250}.
Research Areas Cancer research
Application Images
Description Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p37 antibody [1320] (56253) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Description Immunofluorescence analysis of methanol-fixed MCF7, using p37 [1320](56253) antibody at 1:200 dilution.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:2,000 dilution. A band of 37 kD is detected.

Positive controls: HeLa cell lysate.
References & Data Sheet