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SERPINH1 (11125P-10)

$169.00

Host Quantity Applications Species Reactivity Data Sheet  
10ug WB Human
SKU: 11125P-10 Categories: ,
Overview
Product Name SERPINH1 (11125P-10)
Description Recombinant human SERPINH1 produced in E. coli is a single, non- glycosylated polypeptide containing 439 amino acidsaa 18-417) with a molecular weight of 48.9kDa. It is fused to a 37- amino acid His-tag at the N- terminus.
Target SERPINH1
Species Reactivity Human
Applications WB
Source Escherichia coli
Properties
Form Liquid
Concentration Lot Specific
Formulation Sterile-filtered colorless solution (1mg/ml) in 20mM Tris-HCl, pH 8.0, and 10% glycerol. 
Buffer Formulation 20 mM Tris
Buffer pH pH 8.0
Buffer Cryopreservative 10% glycerol
Purity Greater than 90% as determined by SDS-PAGE
Background SERPINH1 is a member of the serpin superfamily of serine proteinase inhibitors whose expression is induced by heat shock. As a molecular chaperone, it localizes to the endoplasmic reticulum lumen and binds collagen, thus participating in the maturation of collagen molecules, facilitating the folding and assembly of procollagen molecules, retaining unfolded molecules within the ER, and assisting in the transport of correctly folded molecules from the ER to the Golgi apparatus. Autoantibodies to SERPINH1 have been found in cases of rheumatoid arthritis.
Specificity Information
Target Name Serpin H1
Target ID SERPINH1
Alternative Names Hsp47
Sequence Location Endoplasmic reticulum lumen.
Sequence RGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAA EVKKPAAAAA PGTAEKLSPK AATLAERSAG LAFSLYQAMA KDQAVENILV SPVVVASSLG LVSLGGKATT ASQAKAVLSA EQLRDEEVHA GLGELLRSLS NSTARNVTWK LGSRLYGPSS VSFADDFVRS SKQHYNCEHS KINFRDKRSA LQSINEWAAQ TTDGKLPEVT KDVERTDGAL LVNAMFFKPH WDEKFHHKMV DNRGFMVTRS YTVGVMMMHR TGLYNYYDDE KEKLQIVEMP LAHKLSSLII LMPHHVEPLE RLEKLLTKEQ LKIWMGKMQK KAVAISLPKG VVEVTHDLQK HLAGLGLTEA IDKNKADLSR MSGKKDLYLA SVFHATAFEL DTDGNPFDQD IYGREELRSP KLFYADHPFI FLVRDTQSGS LLFIGRLVRP KGDKMRDEL.
Biological Function Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Background SERPINH1 is a member of the serpin superfamily of serine proteinase inhibitors whose expression is induced by heat shock. As a molecular chaperone, it localizes to the endoplasmic reticulum lumen and binds collagen, thus participating in the maturation of collagen molecules, facilitating the folding and assembly of procollagen molecules, retaining unfolded molecules within the ER, and assisting in the transport of correctly folded molecules from the ER to the Golgi apparatus. Autoantibodies to SERPINH1 have been found in cases of rheumatoid arthritis.
Application Images
Additional Information
Handling
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store at or below - 20°C for longer periods of time. Addition of a carrier protein (such as 0.1% HSA or BSA) is recommended for long-term storage.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
References & Data Sheet