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DnaK (11116P-50)

$287.00

Host Quantity Applications Species Reactivity Data Sheet  
50ug WB Human
SKU: 11116P-50 Categories: ,
Overview
Product Name DnaK (11116P-50)
Description Recombinant Human DnaKHsp70) produced in E. coli is a single, non- glycosylated polypeptide chain containing 661 amino acids fused to His-tag at the N-terminus with a total molecular weight of 72.2kDa.
Target DnaK
Species Reactivity Human
Applications WB
Source Escherichia coli
Properties
Form Liquid
Concentration Lot Specific
Formulation Sterile-filtered colorless solution contains 20mM Tris, pH 7.5, and 2mM DTT having a protein concentration of 1mg/ml. 
Buffer Formulation 20 mM Tris
Buffer pH pH 7.5
Purity Greater than 95% as determined by SDS-PAGE and RP- HPLC
Background Hsp70 plays an important role in protein folding and in protecting cells from stress. In most species there are many proteins that belong to the Hsp70 family. Some are expressed only under stress conditions while others are present in cells under normal conditions and are not heat-inducible.
Specificity Information
Target ID DnaK
Alternative Names Hsp70
Sequence MGSSHHHHHH SSGLVPRGSH MAKAAAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPSYVAFTDTERL IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFQVINDGDKPKVQVSYKGD TKAFYPEEIS SMVLTKMKEI AEAYLGYPVT NAVITVPAYF NDSQRQATKDAGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL GGGTFDVSIL TIDDGIFEVKATAGDTHLGG EDFDNRLVNH FVEEFKRKHK KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS TRIPKVQKLL QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLSLGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT KDNNLLGRFELSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK ITITNDKGRL SKEEIERMVQEAEKYKAEDE VQRERVSAKN ALESYAFNMK SAVEDEGLKG KISEADKKKV LDKCQEVISW LDANTLAEKD EFEHKRKELE QVCNPIISGL YQGAGGPGPG GFGAQGPKGG SGSGPTIEEVD
Background Hsp70 plays an important role in protein folding and in protecting cells from stress. In most species there are many proteins that belong to the Hsp70 family. Some are expressed only under stress conditions while others are present in cells under normal conditions and are not heat-inducible.
Application Images
Additional Information
Handling
Storage Although stable at 4°C for 4 weeks, product should be stored at or below -20°C. For long term storage, addition of a carrier protein (0.1% HSA or BSA) is recommended.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
References & Data Sheet