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DnaJ (11111P-25)

$287.00

Host Quantity Applications Species Reactivity Data Sheet  
25ug WB Human
SKU: 11111P-25 Categories: ,
Overview
Product Name DnaJ (11111P-25)
Description Recombinant Human DnaJHsp40) produced in E. coli is a single, non- glycosylated polypeptide chain containing 360 amino acids with a molecular weight of 40.2kDa. This protein includes 6X His tag fused at the N-terminus.
Target DnaJ
Species Reactivity Human
Applications WB
Source Escherichia coli
Properties
Form Liquid
Concentration Lot Specific
Formulation Sterile-filtered colorless solution contains 20mM Tris buffer, pH 8.0, 2mM DTT, 0.2mM PMSF, and 10% glycerol.
Buffer Formulation 20 mM Tris
Buffer pH pH 8.0
Buffer Cryopreservative 10% glycerol
Purity Greater than 95% as determined by SDS-PAGE and RP- HPLC
Background Human Hsp40 is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in protein folding, transport, and assembly. Hsp40 protein contains the J-domain which controls interactions with the ATPase domain of DnaK.
Specificity Information
Target Name DnaJ homolog subfamily B member 1
Target ID DnaJ
Alternative Names Hsp40
Sequence Location Cytoplasm , Nucleus , Nucleus, nucleolus , Note=Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
Sequence MGSSHHHHHH SSGLVPRGSH MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE AYDVLSDPRK REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHAMFAEFFGGRN PFDTFFGQRN GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT KKMKISHKRL NPDGKSIRNE DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK DKPHNIFKRD GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI.
Biological Function Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and rPubMed:24318877, PubMed:9499401}.
Background Human Hsp40 is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in protein folding, transport, and assembly. Hsp40 protein contains the J-domain which controls interactions with the ATPase domain of DnaK.
Application Images
Additional Information
Handling
Storage Although stable at 4°C for 4 weeks, product should be stored at or below -20°C. For long term storage, addition of a carrier protein (0.1% HSA or BSA) is recommended.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
References & Data Sheet