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Cu/Zn Syperoxide Dismutase Monomer (13001P-1000)

$3,660.00

Host Quantity Applications Species Reactivity Data Sheet  
1000ug WB Human
SKU: 13001P-1000 Categories: ,
Overview
Product Name Cu/Zn Syperoxide Dismutase Monomer (13001P-1000)
Description Recombinant Human Cu/Zn SOD1 produced in E. coli is a single monomeric non-glycosylated polypeptide chain containing 154 amino acids with a molecular weight of 15.9kDa.
Target Cu/Zn Syperoxide Dismutase Monomer
Species Reactivity Human
Applications WB
Source Escherichia coli
Properties
Form Liquid
Concentration Lot Specific
Formulation Sterile-filtered solution in 20mM Tris-HCl, pH 7.5 and 10% glycerol.
Buffer Formulation 20 mM Tris
Buffer pH pH 7.5
Buffer Cryopreservative 10% glycerol
Purity Greater than 95% as determined by SDS- PAGE and RP-HPLC
Background Cu/Zn Superoxide Dismutase (SOD1) catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. It protects a cell from dangerous levels of superoxide. SOD1 binds copper and zinc ions and is one of three isozymes responsible for destroying free superoxide radicals. Mutations in SOD1 cause a form of familial amyotrophic lateral sclerosis (ALS).
Specificity Information
Target Name Superoxide dismutase [Cu-Zn]
Target ID Cu/Zn Syperoxide Dismutase Monomer
Alternative Names SOD1
Sequence Location Cytoplasm , Mitochondrion , Nucleus , Note=Predominantly cytoplasmic; the pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
Sequence MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVIGIAQ
Biological Activity Cu/Zn Syperoxide Dismutase Monomer
Biological Function Destroys radicals which are normally produced within the cells and which are toxic to biological systems. {PubMed:24140062}.
Background Cu/Zn Superoxide Dismutase (SOD1) catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. It protects a cell from dangerous levels of superoxide. SOD1 binds copper and zinc ions and is one of three isozymes responsible for destroying free superoxide radicals. Mutations in SOD1 cause a form of familial amyotrophic lateral sclerosis (ALS).
Application Images
Additional Information
Handling
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store at -20°C for longer periods of time. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
References & Data Sheet