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Cardiac Troponin T (24102P-20)

$287.00

Host Quantity Applications Species Reactivity Data Sheet  
20ug WB Human
SKU: 24102P-20 Categories: ,
Overview
Product Name Cardiac Troponin T (24102P-20)
Description Recombinant Human TnT produced in E. coli is a single, non-glycosylated polypeptide containing 305 amino acidsaa 1-285) with a molecular weight of 36.4kDa. It is fused to a 20-amino acid His-tag at the N-terminus.Purity: Greater than 90% as determined by SDS- PAGE
Target Cardiac Troponin T
Species Reactivity Human
Applications WB
Source Escherichia coli
Properties
Form Liquid
Concentration Lot Specific
Formulation Sterile-filtered colorless solution in 20mM Tris, pH 8.0, 10% glycerol. 
Buffer Formulation 20 mM Tris
Buffer pH pH 8.0
Buffer Cryopreservative 10% glycerol
Molecular Mass Molecular weight of 36.4 kDa
Purity Greater than 90% as determined by SDS- PAGE
Background Troponin T (TnT) is the tropomyosin- binding subunit of the troponin complex which is situated on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentrations. Mutations in the TNNT2 gene are associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy and hypertrophic cardiomyopathy.
Specificity Information
Target Name Troponin T, cardiac muscle
Target ID Cardiac Troponin T
Alternative Names TnT
Sequence MGSSHHHHHH SSGLVPRGSH MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN MMHFGGYIQK TERKSGKRQT EREKKKKILA ERRKVLAIDH LNEDQLREKA KELWQSIYNL EAEKFDLQEK FKQQKYEINV LRNRINDNQK VSKTRGKAKV TGRWK
Biological Function Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Background Troponin T (TnT) is the tropomyosin- binding subunit of the troponin complex which is situated on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentrations. Mutations in the TNNT2 gene are associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy and hypertrophic cardiomyopathy.
Application Images
Additional Information
Handling
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store at -20°C for longer periods of time. For long term storage, addition of 0.1% human serum albumin or bovine serum albumin is recommended. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
References & Data Sheet