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Cardiac Troponin I (24001P-50)

$279.00

Host Quantity Applications Species Reactivity Data Sheet  
50ug WB Human
SKU: 24001P-50 Categories: ,
Overview
Product Name Cardiac Troponin I (24001P-50)
Description Recombinant Human TnI produced in E. coli is a single, non-glycosylated polypeptide chain containing 165 amino acids with a molecular weight of 24 kDa.
Target Cardiac Troponin I
Species Reactivity Human
Applications WB
Source Escherichia coli
Properties
Form Liquid
Concentration Lot Specific
Formulation Sterile-filtered solution in 50mM Tris, pH 8.0 and 6M urea.
Buffer Formulation 50mM Tris
Buffer pH pH 8.0
Purity Greater than 98% as determined by SDS- PAGE and RP- HPLC
Background Troponin I (TnI) troponin T (TnT) and troponin C (TnC) form the troponin complex of thin filaments of striated muscle. TnI acts as the inhibitory subunit by blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily consists of 3 genes: TnI- skeletal-fast-twitch, TnI-skeletal-slow- twitch, and TnI-cardiac. The TNNI3 gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissue. Mutations in the TNNI3 gene cause familial hypertrophic cardiomyopathy type 7 and familial restrictive cardiomyopathy.
Specificity Information
Target Name Troponin I, cardiac muscle
Target ID Cardiac Troponin I
Alternative Names TnI
Biological Function Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Background Troponin I (TnI) troponin T (TnT) and troponin C (TnC) form the troponin complex of thin filaments of striated muscle. TnI acts as the inhibitory subunit by blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily consists of 3 genes: TnI- skeletal-fast-twitch, TnI-skeletal-slow- twitch, and TnI-cardiac. The TNNI3 gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissue. Mutations in the TNNI3 gene cause familial hypertrophic cardiomyopathy type 7 and familial restrictive cardiomyopathy.
Application Images
Additional Information
Handling
Storage Although stable at 4°C for 1 week, product is best stored at or below -20°C. Addition of a carrier protein (such as 0.1% HSA or BSA) is recommended for long-term storage.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
References & Data Sheet