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Annexin A1 (13821P-2)

$350.00

Host Quantity Applications Species Reactivity Data Sheet  
2ug WB Human
SKU: 13821P-2 Categories: ,
Overview
Product Name Annexin A1 (13821P-2)
Description Recombinant Human Annexin A1 produced in E. coli is a single, non- glycosylated polypeptide chain containing 346 amino acids with a molecular weight of 38.7kDa.
Target Annexin A1
Species Reactivity Human
Applications WB
Source Escherichia coli
Properties
Form Liquid
Concentration Lot Specific
Formulation Sterile-filtered colorless solution in 20mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM DTT, and 10% glycerol.
Buffer Formulation 20 mM Tris
Buffer pH pH 8.0
Buffer Cryopreservative 10% glycerol
Purity Greater than 95% as determined by SDS-PAGE
Background Annexin A1 is a member of the Ca+2- dependent phospholipid binding proteins that are localized on the cytosolic face of the plasma membrane. It inhibits activity of phospholipase A2, binding 2-4 calcium ions with high affinity. Given the involvement of phospholipase A2 in mediating inflammation, prostaglandins, and leukotrienes, Annexin A1 might have potential anti-inflammatory activity.
Specificity Information
Target Name Annexin A1
Target ID Annexin A1
Alternative Names Annexin A1
Sequence MAMVSEFLKQ AWFIENEEQE YVQTVKSSKG GPGSAVSPYP TFNPSSDVAA LHKAIMVKGV DEATIIDILT KRNNAQRQQI KAAYLQETGK PLDETLKKAL TGHLEEVVLA LLKTPAQFDA DELRAAMKGL GTDEDTLIEI LASRTNKEIR DINRVYREEL KRDLAKDITS DTSGDFRNAL LSLAKGDRSE DFGVNEDLAD SDARALYEAG ERRKGTDVNV FNTILTTRSY PQLRRVFQKY TKYSKHDMNK VLDLELKGDI EKCLTAIVKCATSKPAFFAE KLHQAMKGVG TRHKALIRIM VSRSEIDMND IKAFYQKMYG ISLCQAILDE TKGDYEKILV ALCGGN
Background Annexin A1 is a member of the Ca+2- dependent phospholipid binding proteins that are localized on the cytosolic face of the plasma membrane. It inhibits activity of phospholipase A2, binding 2-4 calcium ions with high affinity. Given the involvement of phospholipase A2 in mediating inflammation, prostaglandins, and leukotrienes, Annexin A1 might have potential anti-inflammatory activity.
Application Images
Additional Information
Handling
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store at or below - 20°C for longer periods of time. For long term storage, addition of a carrier protein (0.1% HSA or BSA) is recommended. For in vitro investigational use only.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
References & Data Sheet