Overview
Product Name Alpha Crystallin A (11075P-100)
Description Recombinant Human CRYAA produced in E. coli is a single, non-glycosylated polypeptide chain containing 173 amino acids with a molecular weight of 19.9kDa.
Target Alpha Crystallin A
Species Reactivity Human
Applications WB
Source Escherichia coli
Properties
Form Liquid
Concentration Lot Specific
Formulation Sterile-filtered colorless solution contains 20mM Tris-HCl, pH 7.5, 50mM NaCl and 1mM EDTA.
Buffer Formulation 20 mM Tris
Buffer pH pH 7.5
Purity Greater than 95% as determined by SDS-PAGE and RP- HPLC
Background Alpha crystallins are composed of two gene products: alpha-A (acidic) and alpha-B (basic). Alpha crystallins are induced by heat shock and are members of the small heat shock protein family. They act as molecular chaperones. In addition, alpha crystallins have autokinase activity and play a role in intracellular architecture. The expression of alpha crystalline A is preferentially restricted to lens cells.
Specificity Information
Target Name Alpha Crystallin A
Target ID Alpha Crystallin A
Alternative Names CRYAA
Sequence MDVTIQHPWF KRTLGPFYPS RLFDQFFGEG LFEYDLLPFL SSTISPYYRQ SLFRTVLDSGISEVRSDRDK FVIFLDVKHF SPEDLTVKVQ DDFVEIHGKH NERQDDHGYI SREFHRRYRLPSNVDQSALS CSLSADGMLT FCGPKIQTGL DATHAERAIP VSREEKPTSA PSS.
Background Alpha crystallins are composed of two gene products: alpha-A (acidic) and alpha-B (basic). Alpha crystallins are induced by heat shock and are members of the small heat shock protein family. They act as molecular chaperones. In addition, alpha crystallins have autokinase activity and play a role in intracellular architecture. The expression of alpha crystalline A is preferentially restricted to lens cells.
Handling
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store at or below -20°C for longer periods of time. For long term storage, addition of a carrier protein (0.1% HSA or BSA) is recommended.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
References & Data Sheet
Data Sheet Download PDF Data Sheet