Anti-Rb Protein Antibody (3102)

$358.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IP,IHC,ELISA Human
SKU: 3102 Categories: , , ,
Overview
Product Name Anti-Rb Protein Antibody (3102)
Description Anti-Rb Protein Mouse Monoclonal Antibody
Target Rb Protein
Species Reactivity Human
Applications WB,IP,IHC,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 4H6
Isotype IgG1
Immunogen Recombinant retinoblastoma
Associated Products
Associated Clone Specificity Notes
Catalog No. Clone No. Amino Acid Specificity ELISA Western Blot Immuno-Precipitation Immuno-Histochemistry

3101

4G2

41-330

+

++

+

++

3102

4H6

121-140

++

++

+

++

3103

7F12

280-300

+

+

+

-

3104

1G5

371-390

++

++

++

++

3105

1E5

511-530

+

++

++

-

3106

1D1

571-590

++

+

+

-

3107

3C8

886-905

+++

+++

+++

+++
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity Recombinant retinoblastoma
Target Name Retinoblastoma-associated protein
Target ID Rb Protein
Uniprot ID P06400
Alternative Names p105-Rb, p110-RB1, pRb, Rb, pp110
Gene Name RB1
Gene ID 5925
Accession Number NP_000312
Sequence Location Nucleus
Biological Function Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). {UniProtKB:P13405, UniProtKB:P33568, PubMed:10499802, PubMed:15084261}.; (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. {PubMed:1316611, PubMed:17974914, PubMed:18701596, PubMed:2839300, PubMed:8892909}.
Research Areas Cancer Research
Application Images
Description Specificity and Applications
Additional Information
Handling
Storage These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
References & Data Sheet
References Wen et al. (1994) J Immunol Meth 169: 231-240.
PMID 7510760