Anti-RAIDD (IN) Antibody (1115)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF Human
SKU: 1115 Categories: , ,
Overview
Product Name Anti-RAIDD (IN) Antibody (1115)
Description Anti-RAIDD (IN) Rabbit Polyclonal Antibody
Target RAIDD (IN)
Species Reactivity Human
Applications ELISA,WB,ICC,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 99- 117 of human RAIDD (accession no. AAB42217).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human RAIDD (22kDa).
Target Name Death domain-containing protein CRADD
Target ID RAIDD (IN)
Uniprot ID P78560
Alternative Names Caspase and RIP adapter with death domain, RIP-associated protein with a death domain
Gene Name CRADD
Gene ID 8738
Accession Number NP_001307028.1
Sequence Location Cytoplasm, Nucleus
Biological Function Adapter protein that associates with PIDD1 and the caspase CASP2 to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis (PubMed:9044836, PubMed:15073321, PubMed:16652156, PubMed:17159900, PubMed:17289572). Also recruits CASP2 to the TNFR-1 signaling complex through its interaction with RIPK1 and TRADD and may play a role in the tumor necrosis factor-mediated signaling pathway (PubMed:8985253). {PubMed:15073321, PubMed:16652156, PubMed:17159900, PubMed:17289572, PubMed:8985253, PubMed:9044836}.
Research Areas Apoptosis
Background A novel cell death adapter was recently identified by two independent laboratories and designated RAIDD (RIP-associated ICH-1/CED-3-homologous protein with DD) and CRADD (caspase and RIP adapter with DD). RAIDD contains a DD and a CARD (caspase recruitment domain) which interact with RIP and caspase, respectively, to transduce death signals. RAIDD is constitutively expressed in many tissues and mediates apoptosis caused by Fas and TNFR1.
Application Images
Description Western blot analysis of RAIDD in whole cell lysates from HeLa (H) or K562 (K) cells with RAIDD antibody at 1:500 dilution.
Description Immunocytochemistry of RAIDD in HeLa cells with RAIDD antibody at 5 ug/mL.
Description Immunofluorescence of RAIDD in Hela cells with RAIDD antibody at 20 ug/mL.
Description Immunofluorescence of RAIDD in Hela cells with RAIDD antibody at 20 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting : use at 1ug/mL.

Positive control: HeLa or K562 cell lysate.

Immunocytochemistry: use at 5ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
References & Data Sheet