Anti-Rab5 Antibody (56415)

$315.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
Rabbit	100ug	WB	Human, Mouse, Rat, Bovine, Monkey

SKU: 56415 Categories: Antibody Products, Neuroscience and Signal Transduction Antibodies, Products

Overview

Product Name Anti-Rab5 Antibody (56415)

Description Anti-Rab5 Rabbit Polyclonal Antibody

Target Rab5

Species Reactivity Human, Mouse, Rat, Bovine, Monkey

Applications WB

Host Rabbit

Clonality Polyclonal

Immunogen Human Rab5 synthetic peptide (identical to canine Rab5) conjugated to KLH

Properties

Form Liquid

Concentration Lot Specific

Formulation PBS, pH 7.4.

Buffer Formulation Phosphate Buffered Saline

Buffer pH pH 7.4

Format Purified

Purification Purified by peptide immuno-affinity chromatography

Specificity This antibody recognizes human, mouse, rat, bovine, and monkey Rab5. Other species have not been tested.

Target Name Ras-related protein Rab-5A

Target ID Rab5

Uniprot ID Q6FI44

Gene Name RAB5A

Research Areas Neuroscience

Background Rab5 is a member of the Rab family, Ras superfamily, of small guanosine triphosphatases (GTPases) and is expressed in all human tissues. Rab GTPases are regulators of membrane trafficking in eukaryotic cells. Their regulatory capacity depends on their ability to cycle between the GDP-bound inactive and GTP-bound active states. Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment. Through these proteins, Rab GTPases regulate vesicle formation, acitn- and tubulin-dependent vesicle movement and membrane fusion. Rabaptin-5 and EEA1 are among the proteins recruited by active Rab5.

Handling

Storage This antibody is stable for at least one (1) year at -20°C.

Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.

Application Instructions Immunoblotting: use at 1ug/ml. A band of ~24-26 kDa is detected.

Positive control: HeLa cell lysate.

Data Sheet