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Anti-PTEN (Phospho-Ser380/Thr382/Thr83) Antibody (43056)

$366.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC,IF Human, Mouse, Rat
SKU: 43056 Categories: ,
Overview
Product Name Anti-PTEN (Phospho-Ser380/Thr382/Thr83) Antibody (43056)
Description Anti-PTEN (Phospho-Ser380/Thr382/Thr83) Rabbit Polyclonal Antibody
Target PTEN (Phospho-Ser380/Thr382/Thr83)
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,IF
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of Serine 380/threonine 382/threonine 383 derived from human PTEN and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human, mouse, and rat PTEN only when phosphorylated at serine 380 and threonine 382/383.Accession no.: P60484, NP_000305.3
Target ID PTEN (Phospho-Ser380/Thr382/Thr83)
Uniprot ID P60484
Post Translational Modification PTEN (Phospho-Ser380/Thr382/Thr83)
Target Phospho-ser380/thr382/thr383
Research Areas Phosphospecific Antibodies
Background Phosphatase and tensin homolog (PTEN) was identified as a tumor suppressor that is mutated in many cancers. PTEN is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin-like domain as well as a catalytic domain similar to the dual specificity protein tyrosine phosphatases. Unlike most protein tyrosine phosphatases, PTEN preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol- 3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating Akt/PKB signaling pathway.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~54kDa is detected.

Immunohistochemistry: use at dilution of 1:50-1:100.

Immunofluorescence: use at dilution of 1:100-1:200.

These are recommended working dilutions.

End user should determine optimal dilutions for their application.
References & Data Sheet