Anti-Progesterone Receptor Antibody (3341)

$503.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
Mouse	100ug	WB	Human

SKU: 3341 Categories: Antibody Products, Cancer Research Antibodies, Products

Overview

Product Name Anti-Progesterone Receptor Antibody (3341)

Description Anti-Progesterone Receptor Mouse Monoclonal Antibody

Target Progesterone Receptor

Species Reactivity Human

Applications WB

Host Mouse

Clonality Monoclonal

Clone ID 4D8

Isotype IgG2a

Immunogen 6-His fusion protein containing the region encoding aa 1-101 of human progesterone receptor expressed in E. coli.

Properties

Form Liquid

Concentration Lot Specific

Formulation PBS, pH 7.4

Buffer Formulation Phosphate Buffered Saline

Buffer pH pH 7.4

Format Purified

Purification Purified by Protein G affinity chromatography

Specificity This antibody recognizes human progesterone receptor (PgR), a member of the steroid family of nuclear receptors. PgR is found as a 94 kD protein (Form A) or a 120 kD protein (Form B). Binding of progesterone to its receptor results in a conformational change that allows dimerization and binding of the receptor to progesterone response elements and activated transcription. When bound to DNA, PgR can positively or negatively regulate gene transcription.

Target Name Progesterone receptor

Target ID Progesterone Receptor

Uniprot ID P06401

Alternative Names PR, Nuclear receptor subfamily 3 group C member 3

Gene Name PGR

Gene ID 5241

Accession Number NP_000917

Sequence Location Nucleus. Cytoplasm. Note=Nucleoplasmic shuttling is both hormone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases.; [Isoform A]: Nucleus. Cytoplasm. Note=Mainly nuclear.; [Isoform 4]: Mitochondrion outer membrane

Biological Function The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as transcriptional activator or repressor. {PubMed:10757795, PubMed:1587864, PubMed:9407067, PubMed:7969170, PubMed:8180103, PubMed:8264658, PubMed:10757795}.; [Isoform B]: Transcriptional activator of several progesteron-dependent promoters in a variety of cell types. Involved in activation of SRC-dependent MAPK signaling on hormone stimulation. {PubMed:7969170}.; [Isoform 4]: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

Research Areas Cancer Research

Handling

Storage This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles.

Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.

Application Instructions Immunoblotting: use at 1-5 ug/ml.

Positive controls: MCF7 cells or recombinant protein.

Data Sheet