Anti-Piccolo Antibody (56484)
$377.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Mouse | 100ug | WB,ELISA | Human, Rat |  |
Overview
Product Name Anti-Piccolo Antibody (56484)
Description Anti-Piccolo Mouse Monoclonal Antibody
Target Piccolo
Species Reactivity Human, Rat
Applications WB,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 6H9-B6
Isotype IgG2b
Immunogen Full-length human Piccolo protein (accession no. BC001304.1)
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human and rat Piccolo isoforms (other species not yet tested). It does not cross-react with Bassoon.
Target Name Protein piccolo
Target ID Piccolo
Uniprot ID Q9QYX7
Alternative Names Aczonin, Brain-derived HLMN protein, Multidomain presynaptic cytomatrix protein
Gene Name Pclo
Sequence Location Cell junction, synapse, presynaptic active zone
Biological Function Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed:19812333). After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (By similarity). At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis (By similarity). Organizes as well the readily releasable pool of synaptic vesicles and safeguards a fraction of them to be not immediately available for action potential-induced release (By similarity). Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy (PubMed:28231469) (By similarity). Mediates also synapse to nucleus communication leading to rUniProtKB:Q9JKS6, PubMed:19812333, PubMed:28231469}.
Research Areas Neuroscience
Background Piccolo is a novel component of the presynaptic cytoskeletal matrix (PCM) assembled at the active zone of neurotransmitter release. Piccolo is a multidomain zinc finger protein structurally related to Bassoon, another PCM protein. Both proteins share components of glutamatergic and GABAergic CNS synapses. Piccolo zinc fingers interact with the dual prenylated rab3A and VAMP2/Synaptobrevin II receptor PRA1. Piccolo is involved in endo- and exocytosis of synaptic vesicles and has been shown to interfere with clathrin- mediated endocytosis by binding to the F- actin and dynamin bind protein Abp1.
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-10ug/ml. A band of ~550kDa is detected
ELISA: use at 1-10ug/ml with Piccolo on the solid phase.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat brain lysate.
ELISA: use at 1-10ug/ml with Piccolo on the solid phase.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat brain lysate.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet