Anti-PfHsp70 Antibody (11147)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ICC/IF P. Facilparum
SKU: 11147 Categories: , , ,
Overview
Product Name Anti-PfHsp70 Antibody (11147)
Description Anti-PfHsp70 Rabbit Polyclonal Antibody
Target PfHsp70
Species Reactivity P. Facilparum
Applications WB,ICC/IF
Host Rabbit
Clonality Polyclonal
Immunogen His-tagged C-terminal amino acids 365-681 of P. falciparum Hsp70 (Accession no. M19753).
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide. Purified by Protein A affinity chromatography.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody recognizes P. falciparum Hsp70.
Target Name Heat shock 70 kDa protein
Target ID PfHsp70
Uniprot ID P11144
Alternative Names HSP70, 74.3 kDa protein, Cytoplasmic antigen
Accession Number M19753
Research Areas Heat Shock& Stress Proteins
Background Plasmodium falciparum expresses two prominent heat shock proteins: heat shock protein 70 (PfHsp70) and heat shock protein 90 (PfHsp90). Both of these proteins have been implicated in the development and pathogenesis of malaria. PfHsp70-1 is the major cytosolic Hsp70 in Plasmodium falciparum. It is abundantly expressed in the blood stages of the parasite and appears to constitute approx. 1-2% of total parasite protein. It is found in P. falciparum in complexes with PfHsp90 and PfHsp40. The transition from the cold blooded mosquito vector to a host represents physiological stress to the parasite, and, additionally, malaria blood stage infection is characterized by intense fever periods. In recent years, it has become clear that heat shock proteins play an essential role during the parasite's life cycle.
Application Images
Description Western blot analysis of Parasite Lysates showing detection of HSP70 protein using Rabbit Anti-HSP70 Polyclonal Antibody (11147). Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (11147) at 1:2000.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.1-1ug/mL. A band of ~70kDa is detected.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet