Anti-pan-Shank Antibody (56468)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,IP,ICC/IF,AM Human, Mouse, Rat
SKU: 56468 Categories: , ,
Overview
Product Name Anti-pan-Shank Antibody (56468)
Description Anti-pan-Shank Mouse Monoclonal Antibody
Target pan-Shank
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,IP,ICC/IF,AM
Host Mouse
Clonality Monoclonal
Clone ID S23b-49
Isotype IgG1
Immunogen Fusion protein corresponding to aa 84-309 (SH3/PDZ domains) of rat Shank2 (accession no. Q9QX74). This sequence is 94
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat Shank 1, 2, and 3.
Target Name SH3 and multiple ankyrin repeat domains protein 2
Target ID pan-Shank
Uniprot ID Q9QX74
Alternative Names Shank2, Cortactin-binding protein 1, CortBP1, GKAP/SAPAP-interacting protein, Proline-rich synapse-associated protein 1, ProSAP1, SPANK-3
Gene Name Shank2
Sequence Location Apical cell membrane, Cytoplasm, Cell junction, synapse, Cell junction, synapse, postsynaptic density, Cell projection, growth cone, Cell projection, dendritic spine
Biological Function Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. {PubMed:10506216}.
Research Areas Neuroscience
Background Shank proteins are a family of scaffold proteins identified through their interaction with a variety of membrane and cyto- plasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are crucial in receptor tyrosine kinase signaling. Recent studies suggest that disruption of glutamate receptors at the Shank-postsynaptic platform could contribute to the destruction of post- synaptic density which underlies synaptic dysfunction and loss in Alzheimer's disease.
Application Images
Description Western Blot analysis of Rat brain membrane lysate showing detection of SHANK protein using Mouse Anti-SHANK Monoclonal Antibody, Clone S23b-49 (56468). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK Monoclonal Antibody (56468) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK (pan) Monoclonal Antibody, Clone S23b-49 (56468). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK (pan) Monoclonal Antibody (56468) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SHANK (pan) Antibody. (D) Composite.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK (pan) Monoclonal Antibody, Clone S23b-49 (56468). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK (pan) Monoclonal Antibody (56468) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK (pan) Antibody (D) Composite.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-10ug/mL. A band of ~160kDa is detected.Immunohistochemistry and

Immunocytochemistry: use at 0.1-1ug/mL

Immunofluorescence: use at 1-10ug/mL

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: Adult rat brain lysate.
References & Data Sheet