Anti-p53DINP1 Antibody (56223)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Human, Mouse, Rat
SKU: 56223 Categories: , ,
Overview
Product Name Anti-p53DINP1 Antibody (56223)
Description Anti-p53DINP1 Rabbit Polyclonal Antibody
Target p53DINP1
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Immunogen Peptide corresponding to aa 1-14 of human p53DINP1.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 0.02% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes alpha and b isoforms of human and mouse p53DINP1.
Target Name Tumor protein p53-inducible nuclear protein 1
Target ID p53DINP1
Uniprot ID Q96A56
Alternative Names Stress-induced protein, p53-dependent damage-inducible nuclear protein 1, p53DINP1
Gene Name TP53INP1
Sequence Location Cytoplasm, cytosol. Nucleus. Nucleus, PML body. Cytoplasmic vesicle, autophagosome. Note=Shuttles between the nucleus and the cytoplasm, depending on cellular stress conditions, and re-localizes to autophagosomes on autophagy activation.
Biological Function Antiproliferative and proapoptotic protein involved in cell stress response which acts as a dual regulator of transcription and autophagy. Acts as a positive regulator of autophagy. In response to cellular stress or activation of autophagy, relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C and regulates autophagy. Acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. Possesses both a p53/TP53-independent intracellular reactive oxygen species (ROS) regulatory function and a p53/TP53-dependent transcription regulatory function. Positively regulates p53/TP53 and p73/TP73 and stimulates their capacity to induce apoptosis and regulate cell cycle. In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis. Acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism. Can reduce cell migration by regulating the expression of SPARC. {PubMed:11511362, PubMed:22421968, PubMed:22470510}.
Research Areas Cancer research
Application Images
Description Western blot analysis of p53DINP1 expression in human lung tissue lysate with p53DINP1 antibody at (A) 0.5 and (B) 1 ug/mL.
Description Immunohistochemical staining of mouse liver using p53DINP1 antibody at 2 ug/mL.
Description Immunofluorescence of p53DINP1 in human liver tissue with p53DINP1 antibody at 5 ug/ml. Green: p53DINP1 antibody (56223)
Red: Phylloidin staining
Blue: DAPI staining
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-1ug/mL

IHC: use at 2ug/mL. Detection of p53DINP1 in mouse liver with #56223 at 2ug/mL.

Immunofluorescence: use at 5-20ugml. Detection of p53DINP1 in human lung tissue lysate with #56223 at (A) 0.5ug/mL and (B) 1ug/mL. Detection of p53DINP1 in human liver with #56223 at 5ug/mL.
References & Data Sheet