Anti-Osteocalcin Antibody (50114)

$509.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ELISA,IHC Human, Rat, Rabbit, Sheep, Goat, Chicken, Canine, Porcine
SKU: 50114 Categories: , ,
Overview
Product Name Anti-Osteocalcin Antibody (50114)
Description Anti-Bovine Osteocalcin Mouse Monoclonal Antibody
Target Osteocalcin
Species Reactivity Human, Rat, Rabbit, Sheep, Goat, Chicken, Canine, Porcine
Applications WB,ELISA,IHC
Host Mouse
Clonality Monoclonal
Clone ID OC4-30
Isotype IgG2a
Immunogen Bovine osteocalcin
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH7.4, containing 1.0% bovine serum albumin, lyophlized.
Buffer Formulation Phosphate Buffered Saline
Buffer Protein Stabilizer 1% Bovine Serum Albumin
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity The g-carboxylated residue at position 17; human, bovine, rat, rabbit, pig, dog, goat, sheep, and chicken.
Target Name Osteocalcin
Target ID Osteocalcin
Uniprot ID P02818
Alternative Names Bone Gla protein, BGP, γ-carboxyglutamic acid-containing protein
Gene Name BGLAP
Sequence Location Secreted.
Biological Function Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Research Areas Hormones/Proteins
Background Osteocalcin is a noncollagenous, 49 amino acid glutamate-rich polypeptide bone matrix protein with a molecular weight of about 5800Da. Osteoblasts produce osteocalcin and incorporate it into the bone matrix. Osteocalcin is released into the circulation from the matrix during bone resorption and, therefore, is considered a marker of bone turnover rather than a specific marker of bone formation.
Application Images
Description Immunohistochemistry: 1-10ug/ml, frozen or paraffin-embedded tissue after proteinase K (0.4m/ml) antigen retrieval. Chondroma: Human bone tumor. Synovial Sarcoma: human osteosarcoma.
Additional Information
Handling
Storage Store stock solution (2. 0 mg/ml) in aliquots at -20 for 1 year, or at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Diluted antibody should not be stored.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Sandwich ELISA: 1-10ug/ml with immobilized antigen, pair with catalog no. 50120.

Immunoblotting: 1-10ug/ml, reducing or non-reducing conditions.

Immunohistochemistry: 1-10ug/ml, frozen or paraffin-embedded tissue after proteinase K (0.4m/ml) antigen retrieval.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet
References Soos et al. (1986) Biochem J 235: 199. Brindle et al. (1990) Biochem J 268: 615. Prigent et al. (1990) J Biol Chem 265: 9970.