Anti-NR1 Glutamate Receptor Antibody (56525)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF Human, Mouse, Rat
SKU: 56525 Categories: , ,
Overview
Product Name Anti-NR1 Glutamate Receptor Antibody (56525)
Description Anti-NR1 Glutamate Receptor Mouse Monoclonal Antibody
Target NR1 Glutamate Receptor
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,ICC/IF
Host Mouse
Clonality Monoclonal
Clone ID S308-48
Isotype IgG1
Immunogen Fusion protein corresponding to aa 42-361 (extracellular N-terminus) of rat NR1 (accession no. NP_058706.1).
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat NR1.
Target Name Glutamate receptor ionotropic, NMDA 1
Target ID NR1 Glutamate Receptor
Uniprot ID P35439
Alternative Names GluN1, Glutamate [NMDA] receptor subunit ζ-1, N-methyl-D-aspartate receptor subunit NR1, NMD-R1
Gene Name Grin1
Sequence Location Cell membrane, Cell junction, synapse, postsynaptic cell membrane, Cell junction, synapse, postsynaptic density
Biological Function Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:1350383, PubMed:1834949, PubMed:1388270, PubMed:8428958, PubMed:18177891, PubMed:28384476, PubMed:15996549, PubMed:24876489, PubMed:27135925, PubMed:27618671, PubMed:28468946). Sensitivity to glutamate and channel kinetics depend on the subunit composition (PubMed:28384476). {PubMed:1350383, PubMed:1388270, PubMed:15996549, PubMed:18177891, PubMed:1834949, PubMed:24876489, PubMed:27135925, PubMed:27618671, PubMed:28384476, PubMed:28468946, PubMed:8428958}.
Research Areas Neuroscience
Background The NMDA receptor (NMDAR), a glutamate receptor, is the predominant molecular device for controlling synaptic plasticity and memory function. NMDAR is a heterotetramer of two NR1 and two NR2 subunits. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN1/NR1 Monoclonal Antibody, Clone S308-48 (56525). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN1/NR1 Monoclonal Antibody (56525) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN1/NR1 Antibody (D) Composite.
Description Western Blot analysis of Rat brain membrane lysate showing detection of NMDAR1 NMDA receptor protein using Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody, Clone S308-48 (56525). Primary Antibody: Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody (56525) at 1:1000.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN1/NR1 Monoclonal Antibody, Clone S308-48 (56525). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN1/NR1 Monoclonal Antibody (56525) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GluN1/NR1 Antibody. (D) Composite.
Additional Information
Handling
Storage This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-2ug/mL. A band of ~105kDa is detected.

Immunohistochemistry: use at 1-5ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.

Positive control: Rat brain membranes.
References & Data Sheet