Anti-NPFF1 Antibody (56110)

$376.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
Rabbit	100ug	WB,ELISA	Human

SKU: 56110 Categories: Antibody Products, Neuroscience and Signal Transduction Antibodies, Products

Overview

Product Name Anti-NPFF1 Antibody (56110)

Description Anti-NPFF1 Rabbit Polyclonal Antibody

Target NPFF1

Species Reactivity Human

Applications WB,ELISA

Host Rabbit

Clonality Polyclonal

Immunogen N-terminal sequence MEGEPSQPPNSSWPLS and C-terminal sequence CSHLPLTIPAWDI of human NPFF1.

Properties

Form Liquid

Concentration Lot Specific

Formulation PBS.

Buffer Formulation Phosphate Buffered Saline

Format Purified

Purification Purified by immunoaffinity chromatography

Specificity This antibody recognizes human NPFF1 (45 kD).

Target Name Neuropeptide FF receptor 1

Target ID NPFF1

Uniprot ID Q9GZQ6

Alternative Names G-protein coupled receptor 147, RFamide-related peptide receptor OT7T022

Gene Name NPFFR1

Sequence Location Cell membrane; Multi-pass membrane protein.

Biological Function Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Research Areas Neuroscience

Background The central nervous system octapeptide, neuropeptide FF (NPFF), is believed to play a role in pain modulation and opiate tolerance. Two G protein-coupled receptors, NPFF1 and NPFF2, have been isolated from human and rat central nervous system tissues. Localization of mRNA and binding sites of these receptors in the dorsal horn of the spinal cord, the lateral hypothalamus, the spinal trigeminal nuclei, and the thalamic nuclei suggest a role for NPFF in pain modulation.

Handling

Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.

Application Instructions Immunoblotting: use at 1:500-1:1,000 dilution

ELISA: use at 1:1,000-1:5,000 dilution.

Data Sheet