Anti-Nitrotyrosine Antibody (56456)

$486.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF,IP,ELISA,FCM,AM Human, Mouse, Rat, Canine
SKU: 56456 Categories: , ,
Overview
Product Name Anti-Nitrotyrosine Antibody (56456)
Description Anti-Nitrotyrosine Mouse Monoclonal Antibody
Target Nitrotyrosine
Species Reactivity Human, Mouse, Rat, Canine
Applications WB,IHC,ICC/IF,IP,ELISA,FCM,AM
Host Mouse
Clonality Monoclonal
Clone ID 39B6
Isotype IgG2a
Immunogen 3-(4-hydroxy-3-nitrophenyl-acetamido) propionic acid conjugated to BSA.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes 3-nitrotyrosine moieties, species-independent. No detectable reactivity with non-nitrated tyrosine.
Target ID Nitrotyrosine
CAS Number 621-44-3
PubChem ID 65124
Research Areas Neuroscience
Background Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. Most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P- 450s, superoxide dismutase, and prostacyclin synthase. Because nitrotyrosine is a stable product of multiple pathways, its plasma concentration may be a useful determinant of NO- dependent damage in vivo. Nitrotyrosine has been detected in inflammatory diseases such as septic shock, rheumatoid arthritis, celiac disease, atherosclerosis, and chronic renal failure.
Application Images
Description Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (56456). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (56456) at 1:25000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.
Description Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (56456). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (56456) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.
Description Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (56456). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (56456) at 1:1000000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x. With anti-microbial.
Description Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (56456). Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (56456) at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red). Courtesy of: Dr. Rajashekhar Gangaraju, University of Indiana, Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute.
Description Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (56456). Tissue: liver tissue . Species: Rat. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (56456) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green).
Description Western Blot analysis of Human A549 cells showing detection of Multiple Bands Nitrotyrosine protein using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (56456). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (56456) at 1:1000 for 2.5 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1.0ug/mL.

Positive control: Linsidomine (SIN-1)-treated BSA.

Immunohistochemistry: use at 1-5ug/mL.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet