Anti-Nitric Oxide Synthase, Neuronal Antibody (56116)

$376.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
Rabbit	100ug	WB	Human

SKU: 56116 Categories: Antibody Products, Neuroscience and Signal Transduction Antibodies, Products

Overview

Product Name Anti-Nitric Oxide Synthase, Neuronal Antibody (56116)

Description Anti-Neuronal NO Synthase Rabbit Polyclonal Antibody

Target Nitric Oxide Synthase, Neuronal

Species Reactivity Human

Applications WB

Host Rabbit

Clonality Polyclonal

Immunogen Peptide corresponding to aa 19-31 of human neuronal nitric oxide synthase.

Properties

Form Liquid

Concentration Lot Specific

Formulation PBS, pH 7.4.

Buffer Formulation Phosphate Buffered Saline

Buffer pH pH 7.4

Format Purified

Purification Purified by immunoaffinity chromatography

Specificity This antibody recognizes the human variant of neuronal nitric oxide synthase (nNOS). nNOS is one of three NOS isoforms along with endothelial cell NOS (eNOS) and inducible NOS (iNOS). The three isoforms differ in primary sequence, having only 50-60% sequence identity. nNOS and iNOS can be purified from cytosol while eNOS is membrane-bound. All three NOS isoforms catalyze the formation of NO and L-citrulline from L-arginine.

Target Name Nitric oxide synthase, brain

Target ID Nitric Oxide Synthase, Neuronal

Uniprot ID P29475

Alternative Names EC 1.14.13.39, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, N-NOS, nNOS, Peptidyl-cysteine S-nitrosylase NOS1, bNOS

Gene Name NOS1

Sequence Location Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine

Biological Function Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

Research Areas Neuroscience

Handling

Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.

Application Instructions Immunoblotting: use at 1:500-1:1,000 dilution. In immunoblots, a band of approximately 155 kD is detected.

Data Sheet