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Anti-NIK (CT) Antibody (1129)

$478.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IF,ICC Human
SKU: 1129 Categories: , ,
Overview
Product Name Anti-NIK (CT) Antibody (1129)
Description Anti-NIK (CT) Rabbit Polyclonal Antibody
Target NIK (CT)
Species Reactivity Human
Applications ELISA,WB,IF,ICC
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthetic peptide corresponding to aa 931-947 of human NIK (accession no. Q99558).
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, 1mg/ml, pH 7.4, 0.02% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human NIK.
Target Name Mitogen-activated protein kinase kinase kinase 14
Target ID NIK (CT)
Uniprot ID Q99558
Alternative Names EC 2.7.11.25, NF-κ-β-inducing kinase, HsNIK, Serine/threonine-protein kinase NIK
Gene Name MAP3K14
Gene ID 9020
Accession Number NP_003945.2
Sequence Location Cytoplasm.
Biological Function Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner. {PubMed:15084608}.
Research Areas Neuroscience
Background Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha, LPS, and mitogens. A serine/threonine protein kinase which mediates NF-kappaB activation by IL-1, TNFalpha, and CD95 has been identified and designated NIK (for NF-kappaB inducing kinase). NIK is an activator of IkappaB kinase alpha and beta (IKKalpha and IKKbeta). Therefore, NIK is a key molecule in the NF-kappaB signaling pathway leading to the expression of a variety of gene products in response to pro-inflammatory cytokines and bacterial products.
Application Images
Additional Information
Handling
Storage This antibody is stable for 3 months at 4°C and at least 1 year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: Recommended concentration: 2-4ug/ml.

Optimal dilution should be determined by End user.

Positive control: Whole cell lysate of NIK-transfected 293 cells.
References & Data Sheet