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Anti-NEI3 Antibody (12523)

$384.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB Human
SKU: 12523 Categories: , ,
Overview
Product Name Anti-NEI3 Antibody (12523)
Description Anti-NE13 Rabbit Polyclonal Antibody
Target NEI3
Species Reactivity Human
Applications WB
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to amino acids 164-177 (LRAESEVKKQKGRMLG) of human NEI3 conjugated to KLH at the C- terminus.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human NEI3 protein. Sequence homology to mouse and rat is 85%.
Target Name Endonuclease 8-like 3
Target ID NEI3
Uniprot ID Q8TAT5
Alternative Names EC 3.2.2.-, EC 4.2.99.18, DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Endonuclease VIII-like 3, Nei-like protein 3
Gene Name NEIL3
Accession Number NP_060718
Sequence Location Nucleus, Chromosome
Biological Function DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA) (PubMed:12433996, PubMed:19170771, PubMed:22569481, PubMed:23755964). Mediates interstrand cross-link repair in response to replication stress: acts by mediating DNA glycosylase activity, cleaving one of the two N-glycosyl bonds comprising the interstrand cross-link, which avoids the formation of a double-strand break but generates an abasic site that is bypassed by translesion synthesis polymerases (By similarity). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also rUniProtKB:A0A1L8HU22, PubMed:12433996, PubMed:19170771, PubMed:22569481, PubMed:23755964}.
Research Areas DNA
Background Prokaryotes and lower eukaryotes possess redundant activities that remove the oxidative DNA base damages produced during normal oxidative metabolism and which have been associated with cancer and aging. Thus far, only one oxidized pyrimidine- specific DNA glycosylase has been identified in humans, hNthl. NEI3 is one of three new putative human DNA glycosylases.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500 dilution. A band of approximately 68 kDa is detected.

Positive control: human testes tissue lysate.
References & Data Sheet
References Bandaru V et al. (2002) DNA Repair 1:517-29.