Anti-Myelin Basic Protein Antibody (24201)

$635.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 500ug WB,IHC,ELISA Human, Mouse, Rat, Rabbit, Sheep, Goat, Primate
SKU: 24201 Categories: , ,
Overview
Product Name Anti-Myelin Basic Protein Antibody (24201)
Description Anti-Myelin Basic Protein Mouse Monoclonal Antibody
Target Myelin Basic Protein
Species Reactivity Human, Mouse, Rat, Rabbit, Sheep, Goat, Primate
Applications WB,IHC,ELISA
Host Mouse
Clonality Monoclonal
Clone ID MBP101
Isotype IgG2b
Immunogen Purified human myelin basic protein.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody reacts with residues 130-136 in human myelin basic protein (21.5kDa and 18.5kDa molecular forms). It also recognizes primate, rabbit, sheep, goat, rat, and mouse myelin basic protein.
Target Name Myelin basic protein
Target ID Myelin Basic Protein
Uniprot ID P02686
Alternative Names MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein
Gene Name MBP
Sequence Location Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.; [Isoform 3]: Nucleus
Biological Function The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. {PubMed:8544862}.
Research Areas Neuroscience
Application Images
Description ELISA: Human myelin basic protein coated on ELISA wells at 5ug/ml; binding detected with HRP-conjugated goat anti-mouse IgG + ABTS substrate.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions This antibody may be used in ELISA, immunohistochemistry, and Western blot procedures for detection and/or quantitation of myelin basic protein.ELISA: Human myelin basic protein coated on ELISA wells at 5ug/ml; binding detected with HRP-conjugated goat anti-mouse IgG + ABTS substrate.
References & Data Sheet