Anti-Mouse IgG H&L Chain Antibody, HRP conjugate (80101)

$231.00

Host Quantity Applications Species Reactivity Data Sheet  
Goat 1mg WB,IHC,ICC,ELISA Mouse
SKU: 80101 Categories: , ,
Overview
Product Name Anti-Mouse IgG H&L Chain Antibody, HRP conjugate (80101)
Description Anti-Mouse IgG H&L Chain, Peroxidase Goat Polyclonal Antibody
Target Mouse IgG H&L Chain
Species Reactivity Mouse
Applications WB,IHC,ICC,ELISA
Host Goat
Clonality Polyclonal
Isotype Whole IgG
Conjugate HRP
Immunogen Mouse IgG
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Buffer Formulation Phosphate Buffered Saline
Buffer Anti-Microbial 0.1% Pro-Clean 400
Buffer Protein Stabilizer 0.2% Bovine Serum Albumin
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. Reactivity with non- immunoglobulin serum proteins is not detected. This antibody may cross-react with IgG from other species.
Target ID Mouse IgG H&L Chain
Research Areas Secondary Antibodies
Application Images
Description Detection of human p53 by immunoperoxidase. Samples: FFPE serial sections of human stomach carcinoma. Primary Antibody: mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: goat anti-mouse IgG-heavy and light chain Antibody HRP Conjugated (80101 Lot 33) used at a dilution of 1:200 (5µg/ml). Detection: DAB
Additional Information
Handling
Storage Store at 2-8°C. Shelf life is one (1) year from date of receipt.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions ELISA: 1:1,000-1:100,000

Western Blot: 1:1,000-1:30,000

Immunohistochemistry: 1:200-1:500

Immunocytochemistry: 1:200-1:500.

Prepare working dilutions immediately before use.

These are recommended dilutions. User should determine optimal dilutions for their applications.
References & Data Sheet