Anti-Measles Nucleoprotein Antibody (90018)
$520.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Mouse | 100ug | WB,ICC,ELISA | Measles Virus |  |
Overview
Product Name Anti-Measles Nucleoprotein Antibody (90018)
Description Anti-Measles Nucleoprotein Mouse Monoclonal Antibody
Target Measles Nucleoprotein
Species Reactivity Measles Virus
Applications WB,ICC,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 2F3
Isotype IgG1
Immunogen Recombinant Measles virus nucleoprotein.
Properties
Form Lyophilized
Formulation Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
Buffer Formulation Tris Buffer
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody reacts with Measles virus nucleoprotein.
Target Name Nucleoprotein
Target ID Measles Nucleoprotein
Uniprot ID Q89933
Alternative Names Nucleocapsid protein, NP, Protein N
Gene Name N
Sequence Location Virion, Host cytoplasm.
Biological Function Encapsidates the genome in a ratio of 1 N per 6 ribonucleotides, protecting it from nucleases. The nucleocapsid (NC) has a helical structure with either 12.35 or 11.64 N per turn, approximately 20 nm in diameter, with a hollow central cavity approximately 5 nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest.
Research Areas Infectious Disease
Background Measles virus (MeV) is an enveloped RNA virus in the Morbillivirus genus of the Paramyxoviridae family. Its non- segmented, negative-sense, single- stranded RNA genome is encapsidated by the viral nucleoprotein (N) within a helical nucleocapsid. Transcription and replication are carried out on this N:RNA complex by the viral polymerase complex which consists of two components, the large protein (L) and the phosphoprotein (P). MeV N consists of two regions: a structured N-terminal moiety, NCORE (aa 1–400), which contains all the regions necessary for self-assembly and RNA-binding, and a C-terminal domain, NTAIL(aa 401–525) that is intrinsically unstructured and is exposed at the surface of the viral nucleocapsid.
Handling
Storage This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
Dilution Instructions Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.
Application Instructions ELISA: use at 0.1-1.0ug/ml
Immunoblotting:
User should use at 1-5ug/ml. A band of ~60kDa is detected
Immunocytochemistry: use at 2- 10ug/ml on virus-infected cells.
These are recommended concentrations.
End user should determine optimal concentrations for their applications.
Immunoblotting:
User should use at 1-5ug/ml. A band of ~60kDa is detected
Immunocytochemistry: use at 2- 10ug/ml on virus-infected cells.
These are recommended concentrations.
End user should determine optimal concentrations for their applications.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet