Anti-LRP4 (extracellular) Antibody (56540)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF Mouse, Rat
SKU: 56540 Categories: , ,
Overview
Product Name Anti-LRP4 (extracellular) Antibody (56540)
Description Anti-LRP4 (extracellular) Mouse Monoclonal Antibody
Target LRP4 (extracellular)
Species Reactivity Mouse, Rat
Applications WB,ICC/IF
Host Mouse
Clonality Monoclonal
Clone ID S207-27
Isotype IgG2a
Immunogen Fusion protein corresponding to aa 26-350 (extracellular N-terminus) of mouse LRP4 (accession no.Q8V156).
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes mouse and rat LRP4.
Target Name Low-density lipoprotein receptor-related protein 4
Target ID LRP4 (extracellular)
Uniprot ID Q8VI56
Alternative Names LRP-4, LDLR dan
Gene Name Lrp4
Sequence Location Cell membrane, Single-pass type I membrane protein
Biological Function Mediates SOST-dependent inhibition of bone formation. Functions as a specific facilitator of SOST-mediated inhibition of Wnt signaling. Plays a key role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Directly binds AGRIN and recruits it to the MUSK signaling complex. Mediates the AGRIN-induced phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Alternatively, may be involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. More generally, has been proposed to function as a cell surface endocytic receptor binding and internalizing extracellular ligands for degradation by lysosomes. Plays an essential role in the process of digit differentiation (PubMed:16517118). {PubMed:16517118, PubMed:18848351, PubMed:21471202}.
Research Areas Neuroscience
Background The formation of neuromuscular junctions (NMJ) requires the interaction between motor neurons and muscle fibers. LRP4 (low-density lipoprotein receptor-related protein 4) is a receptor of agrin that is thought to act in cis to stimulate MuSK in muscle fibers for postsynaptic differentiation. Recent studies of muscle- specific mutants suggest that LRP4 is involved in determining where AChR clusters form in muscle fibers, postsynaptic differentiation, and axon terminal development.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRP4 Monoclonal Antibody, Clone S207-27 (56540). Tissue: COS cells transfected with 2 ug human LRP4 plasmid. Species: Human. Fixation: 4% PFA for 10 min. Primary Antibody: Mouse Anti-LRP4 Monoclonal Antibody (56540) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-mouse: Alexa 488. Counterstain: DAPI.
Description Western Blot analysis of Rat brain membrane lysate showing detection of LRP4 protein using Mouse Anti-LRP4 Monoclonal Antibody, Clone S207-27 (56540). Primary Antibody: Mouse Anti-LRP4 Monoclonal Antibody (56540) at 1:500, 1:1000, and 1:2000.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRP4 (Extracellular) Monoclonal Antibody, Clone S207-27 (56540). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-LRP4 (Extracellular) Monoclonal Antibody (56540) at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LRP4 (Extracellular) Antibody (D) Composite.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRP4 (Extracellular) Monoclonal Antibody, Clone S207-27 (56540). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LRP4 (Extracellular) Monoclonal Antibody (56540) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) LRP4 (Extracellular) Antibody. (D) Composite.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL. Predicted molecular weight is ~215kDa. Degradation products of approx. 150kDa are also detected.

Positive control: rat brain lysate.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet