Anti-Lactoferrin Antibody (21101)

$235.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
Mouse	100ug	WB,ELISA	Human

SKU: 21101 Categories: Antibody Products, Hormones and Serum Protein Antibodies, Products

Overview

Product Name Anti-Lactoferrin Antibody (21101)

Description Anti-Lactoferrin Mouse Monoclonal Antibody

Target Lactoferrin

Species Reactivity Human

Applications WB,ELISA

Host Mouse

Clonality Monoclonal

Clone ID 2B8

Isotype IgG1

Immunogen Purified lactoferrin from human milk.

Properties

Form Liquid

Concentration Lot Specific

Formulation PBS containing 0.1% sodium azide

Buffer Formulation Phosphate Buffered Saline

Buffer Anti-Microbial 0.1% Sodium Azide

Format Purified

Purification Purified by Protein A affinity chromatography

Specificity This antibody reacts in Western blots of whole serum proteins with a lactoferrin band of 77kD. No cross-reactivity with other human serum proteins.

Target Name Lactotransferrin

Target ID Lactoferrin

Uniprot ID P02788

Alternative Names Lactoferrin, EC 3.4.21.-, Growth-inhibiting protein 12, Talalactoferrin [Cleaved into: Lactoferricin-H

Gene Name LTF

Sequence Location [Isoform 1]: Secreted. Cytoplasmic granule. Note=Secreted into most exocrine fluids by various endothelial cells. Stored in the secondary granules of neutrophils.; [Isoform δLf]: Cytoplasm. Nucleus. Note=Mainly localized in the cytoplasm.

Biological Function Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. {PubMed:22900286}.; [Lactotransferrin]: Major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions (PubMed:14573629, PubMed:1599934, PubMed:6802759, PubMed:3169987, PubMed:11179314, PubMed:12693969). Has antimicrobial activity, which depends on the extracellular cation concentration (PubMed:6802759). Antimicrobial properties include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane (PubMed:14573629, PubMed:1599934, PubMed:6802759, PubMed:3169987, PubMed:11179314, PubMed:12693969). Can also prevent bacterial biofilm development in P.aeruginosa infection (PubMed:12037568). Has weak antifungal activity against C.albicans (PubMed:11083624). Has anabolic, differentiating and anti-apoptotic effects on osteoblasts and can also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth (PubMed:15166119). Promotes binding of species C adenoviruses to epithelial cells, promoting adenovirus infection (PubMed:17079302). Can inhibit papillomavirus infections (PubMed:17481742). Stimulates the TLR4 signaling pathway leading to NF-kappa-B activation and subsequent pro-inflammatory cytokine production while also interfering with the lipopolysaccharide (LPS)-stimulated TLR4 signaling (PubMed:20345905). Inhibits neutrophil granulocyte migration to sites of apoptosis, when secreted by apoptotic cells (PubMed:19033648). Stimulates VEGFA-mediated endothelial cell migration and proliferation (PubMed:16842782). Binds heparin, chondroitin sulfate and possibly other glycosaminoglycans (GAGs) (PubMed:9359845). Also binds specifically to pneumococcal surface protein A (PspA), the lipid A portion of bacterial lipopolysaccharide (LPS), lysozyme and DNA (PubMed:9359845). {PubMed:11083624, PubMed:11179314, PubMed:12037568, PubMed:12693969, PubMed:14573629, PubMed:15166119, PubMed:1599934, PubMed:16842782, PubMed:17079302, PubMed:17481742, PubMed:19033648, PubMed:20345905, PubMed:3169987, PubMed:6802759, PubMed:9359845}.; Lactoferricin binds to the bacterial surface and is crucial for the bactericidal functions. Has some antiviral activity against papillomavirus infection (PubMed:17481742). N-terminal region shows strong antifungal activity against C.albicans (PubMed:11083624). Contains two BBXB heparin-binding consensus sequences that appear to form the predominate functional GAG-binding site. {PubMed:11083624, PubMed:17481742}.; [Kaliocin-1]: Has antimicrobial activity and is able to permeabilize different ions through liposomal membranes. {PubMed:12693969}.; [Lactoferroxin-A]: Has opioid antagonist activity (PubMed:1369293). Shows preference for mu-receptor (PubMed:1369293). {PubMed:1369293}.; [Lactoferroxin-B]: Has opioid antagonist activity (PubMed:1369293). Shows higher degrees of preference for kappa-receptors than for mu-receptors (PubMed:1369293). {PubMed:1369293}.; [Lactoferroxin-C]: Has opioid antagonist activity (PubMed:1369293). Shows higher degrees of preference for kappa-receptors than for mu-receptors (PubMed:1369293). {PubMed:1369293}.; The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions (PubMed:12535064). This function contributes to the antimicrobial activity (PubMed:12535064). Shows a preferential cleavage at -Arg-Ser-Arg-Arg-|- and -Arg-Arg-Ser-Arg-|-, and of Z-Phe-Arg-|-aminomethylcoumarin sites (PubMed:12535064). {PubMed:12535064}.; [Isoform DeltaLf]: Transcription factor with antiproliferative properties and ability to induce cell cycle arrest (PubMed:15222485). Binds to the DeltaLf response element found in the SKP1, BAX, DCPS, and SELENOH promoters (PubMed:22320386). {PubMed:15222485, PubMed:22320386}.

Research Areas Hormones/Proteins

Handling

Storage These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.

Application Instructions This antibody may be used in immuno-assays to detect and quantitate human lactoferrin. Not all applications have been investigated.

Data Sheet

Anti-Lactoferrin Antibody (21101)

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