Anti-IRAK2 Antibody (2123)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF Human
SKU: 2123 Categories: , ,
Overview
Product Name Anti-IRAK2 Antibody (2123)
Description Anti-IRAK2 Rabbit Polyclonal Antibody
Target IRAK2
Species Reactivity Human
Applications ELISA,WB,ICC,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 546- 564 of human IRAK2.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes full-length human IRAK2 (65 kD).
Target Name Interleukin-1 receptor-associated kinase-like 2
Target ID IRAK2
Uniprot ID O43187
Alternative Names IRAK-2
Gene Name IRAK2
Gene ID 3656
Accession Number NP_001561
Biological Function Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. {PubMed:10383454, PubMed:9374458}.
Research Areas Neuroscience
Background The pro-inflammatory cytokine IL-1 induces cellular responses through two subunits of its receptor, IL-1 receptor 1 (IL-1R1) and IL- 1 receptor accessory protein (IL-1RacP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kappaB, a key transcription factor that mediates inflammatory and immune respones. A novel member of the IRAK/Pelle family was recently identified and designated IRAK2. Both IRAK and IRAK2 associate with the subunits of the IL-1R complex after IL-1 binding and lead to NF-kappaB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kappaB activation. Members of the IRAK/Pelle family play a central role in IL-1R and TLR- mediated inflammatory responses. IRAK2 is expressed in a variety of human tissues.
Application Images
Description Western blot analysis of IRAK2 in HeLa (H) and K562 (K) whole cell lysate with IRAK2 antibody (C2) at 2 ug/mL.
Description Immunocytochemical staining of HeLa cells using IRAK2 antibody at 2 ug/mL.
Description Immunofluorescence of IRAK-2 in Hela cells with IRAK-2 antibody at 10 ug/mL.
Description Immunofluorescence of IRAK-M in human spleen tissue with IRAK M antibody at 20 ug/mL. Green: IRAK-2 Antibody (2123)
Blue: DAPI staining
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500-1:1,000 dilution.

Positive control: Whole cell lysate from HeLa cells or K562 cells. No cross- reactivity with IRAK.
References & Data Sheet