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Anti-Integrin -β3 (Phospho-Tyr773) Antibody (43060)

$366.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC Human, Mouse, Rat
SKU: 43060 Categories: ,
Overview
Product Name Anti-Integrin -β3 (Phospho-Tyr773) Antibody (43060)
Description Anti-Integrin -β3 (Phospho-Tyr773) Rabbit Polyclonal Antibody
Target Integrin -β3 (Phospho-Tyr773)
Species Reactivity Human, Mouse, Rat
Applications WB,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of tyrosine 773 (P-L-Y(p)-K-E) derived from human Integrin b3.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human, mouse, and rat Integrin b3 only when phosphorylated at tyrosine 773.
Target ID Integrin -β3 (Phospho-Tyr773)
Post Translational Modification Integrin -β3 (Phospho-Tyr773)
Target Phospho-tyr773
Research Areas Phosphospecific Antibodies
Background The Integrin beta3 (ITGB3) gene encodes glycoprotein IIIa (GP IIIa), the beta subunit of the platelet membrane adhesive protein receptor complex GP IIb/IIIa. The alpha subunit, GP IIb, is encoded by the ITGA2B gene. The GP IIb/IIIa complex belongs to the integrin class of cell adhesion molecule receptors that share a common heterodimeric structure with alpha and beta subunits. Glycoprotein IIIa is also the beta subunit of 2 other integrins, fibronectin receptor and vitronectin receptor which have distinctive alpha subunits.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~110kDa is detected.

Immunohistochemistry: use at dilution of 1:50- 1:100.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications.
References & Data Sheet