Anti-Insulin Growth Factor-1 Receptor (IGF-1R) Antibody (35103)

$375.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
Mouse	100ug	WB	Human

SKU: 35103 Categories: Antibody Products, Growth Factors/Cytokines/Receptor Antibodies, Products

Overview

Product Name Anti-Insulin Growth Factor-1 Receptor (IGF-1R) Antibody (35103)

Description Anti-IGF-1 receptor Mouse Monoclonal Antibody

Target Insulin Growth Factor-1 Receptor (IGF-1R)

Species Reactivity Human

Applications WB

Host Mouse

Clonality Monoclonal

Clone ID 2C8

Isotype IgG1

Immunogen Purified human placental IGF-1 receptors.

Properties

Form Liquid

Concentration Lot Specific

Formulation PBS, pH 7.4

Buffer Formulation Phosphate Buffered Saline

Buffer pH pH 7.4

Format Purified

Purification Purified by Protein G affinity chromatography

Specificity All three clones are specific for the human IGF-1 receptor; 35102 and 35103 recognize the alpha subunit: 35101 (3B7) stimulates binding of IGF-1 and -2; stimulates basal and IGF- 1 stimulated cell growth. 35102 (1H7) inhibits binding of IGF-1 and -2; inhibits cell growth. 35103 (2C8) has no effect on binding of IGF-1 or -2.

Target Name Insulin-like growth factor 1 receptor

Target ID Insulin Growth Factor-1 Receptor (IGF-1R)

Uniprot ID P08069

Alternative Names EC 2.7.10.1, Insulin-like growth factor I receptor, IGF-I receptor, CD antigen CD221 [Cleaved into: Insulin-like growth factor 1 receptorα chain; Insulin-like growth factor 1 receptor β chain]

Gene Name IGF1R

Sequence Location Cell membrane, Single-pass type I membrane protein

Biological Function Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R.; When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.

Research Areas Growth Factors, Cytokines, Receptors

Handling

Storage These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.

Application Instructions Use these antibodies in research studies to delineate signal transduction pathways specific for IGF's and for detecting IGF-1 receptors in crude samples. 35101 may be used to immunoprecipitate IGF-1 receptors. 35102 and 35103 can recognize the IGF-1 a subunit on immunoblots.

References Xiong et al. (1992) Proc Natl Acad Sci USA 89: 5356
Li et al. (1993) Biochem Biophys Res Commun 196: 92.

PMID 8216340
8216340

Data Sheet