Anti-IKKβ Antibody (2121)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF Human
SKU: 2121 Categories: , ,
Overview
Product Name Anti-IKKβ Antibody (2121)
Description Anti-Ikk β Rabbit Polyclonal Antibody
Target IKKβ
Species Reactivity Human
Applications ELISA,WB,ICC,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 662-680 of human IKKb which differs from the corresponding murine sequence by one amino acid.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human IKKb (87 kD). No cross-reactivity with IKKalpha or IKKg.
Target Name Inhibitor of nuclear factor κ-B kinase subunit β
Target ID IKKβ
Uniprot ID O14920
Alternative Names I-κ-B-kinase β, IKK-B, IKK-β, IkBKB, EC 2.7.11.10, I-κ-B kinase 2, IKK2, Nuclear factor NF-κ-B inhibitor kinase β, NFKBIKB, Serine/threonine protein kinase IKBKB, EC 2.7.11.1
Gene Name IKBKB
Gene ID 3551
Accession Number NP_001547
Sequence Location Cytoplasm, Nucleus, Membrane raft
Biological Function Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses (PubMed:30337470). Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE (PubMed:11297557, PubMed:20410276). IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor (PubMed:15084260). Also phosphorylates other substrates including NCOA3, BCL10 and IRS1 (PubMed:17213322). Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation (PubMed:11297557). Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death (By similarity). Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus (PubMed:25326418). {UniProtKB:O88351, PubMed:11297557, PubMed:15084260, PubMed:17213322, PubMed:19716809, PubMed:20410276, PubMed:20434986, PubMed:20797629, PubMed:21138416, PubMed:25326418, PubMed:30337470}.
Research Areas Neuroscience
Background Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and key mediator of gene expression during immune and inflammatory responses. NF-kappaB activates numerous genes in response to extracellular stimuli, such as IL-1, TNFalpha, and LPS. NF-kappaB is associated with IkappaB in cytoplasm, which inhibits NF-kappaB activity. IkappaB kinase (IKK), which phosphorylates IkappaB and mediates IkappaB degradation and NF-kappaB activation, was recently identified. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKalpha and IKKbeta). IKKalpha and IKKbeta interact with each other, and both are essential for NF-B activation. IKKbeta phosphorylates both IkappaB-alpha and IkappaB-beta and is expressed in a variety of human tissues.
Application Images
Description Western blot analysis of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.
Description Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 ug/mL.
Description Immunofluorescence of IKK beta in Hela cells with IKK beta antibody at 10 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500-1:1,000 dilution.

Positive control: Whole cell lysate from Jurkat cells.
References & Data Sheet