Anti-CCR5 (NT) Antibody (1112)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Human
SKU: 1112 Categories: , ,
Overview
Product Name Anti-CCR5 (NT) Antibody (1112)
Description Anti-CCR5 (NT) Rabbit Polyclonal Antibody
Target CCR5 (NT)
Species Reactivity Human
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 6-20 of human CCR5.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human CCR5 (40 kD).
Target Name C-C chemokine receptor type 5
Target ID CCR5 (NT)
Uniprot ID P51681
Alternative Names C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD antigen CD195
Gene Name CCR5
Gene ID 1234
Accession Number NP_000570
Sequence Location Cell membrane, Multi-pass membrane protein
Biological Function Receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Participates in T-lymphocyte migration to the infection site by acting as a chemotactic receptor (PubMed:30713770). {PubMed:10383387, PubMed:11323418, PubMed:30713770, PubMed:8639485, PubMed:8663314, PubMed:8699119}.; (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) of human immunodeficiency virus-1/HIV-1. {PubMed:10383387, PubMed:21763489, PubMed:8649511, PubMed:8649512, PubMed:9632396}.
Research Areas Infectious Disease
Background Human immunodeficiency virus (HIV) and related viruses require coreceptors in addition to CD4 to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b, CCR8, GPR15, Bonzo, GPR1, and V28, have been identified as HIV coreceptors. Among them, CCR5 is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains. CCR5 is required for infection by HIV-1, HIV-2, and SIV. The beta-chemokines RANTES, MIP-1alpha, and MIP-1beta are the ligands for CCR5 and prevent infection by macrophage-tropic HIV-1. CCR5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV binding sites. Messenger RNA for CCR5 is expressed in lymphoid cells and tissues.
Application Images
Description Western blot analysis of CCR5 in THP-1 whole cell lysate with CCR5 antibody at 1 ug/mL.
Description Immunohistochemistry of CCR5 in human lymph node tissue with CCR5 antibody at 20 ug/mL.
Description Immunohistochemistry of CCR5 in human lymph node tissue with CCR5 antibody at 2.5 ug/ml.
Description Immunofluorescence of CCR5 in human lymph node tissue with CCR5 antibody at 20 ug/mL. Green: CCR5 Antibody (1112)
Blue: DAPI staining
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:1,000-1:2,000 dilution.

Positive control: Whole cell lysate from THP-1 cells.
References & Data Sheet