Anti-Glucocorticoid Receptor Antibody (36101)
$496.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Sheep | 200ul | WB | Human, Monkey |  |
Overview
Product Name Anti-Glucocorticoid Receptor Antibody (36101)
Description Anti-Glucocorticoid Receptor Sheep Polyclonal Antibody
Target Glucocorticoid Receptor
Species Reactivity Human, Monkey
Applications WB
Host Sheep
Clonality Polyclonal
Immunogen Peptide consisting of amino acids 150-175 of squirrel monkey glucocorticoid receptor coupled to diptheria toxoid. This sequence is identical in human glucocorticoid receptor except for 2 amino acids (underlined):
Properties
Form Liquid
Concentration Lot Specific
Formulation Whole antiserum
Buffer Formulation Whole Antiserum
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity This antibody reacts specifically with monkey and human glucocorticoid receptor.
Target Name Glucocorticoid receptor
Target ID Glucocorticoid Receptor
Uniprot ID P04150
Alternative Names GR, Nuclear receptor subfamily 3 group C member 1
Gene Name NR3C1
Sequence Location [Isoform α]: Cytoplasm, Nucleus, Mitochondrion, Cytoplasm, cytoskeleton, spindle, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Biological Function Receptor for glucocorticoids (GC) (PubMed:27120390). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity). {UniProtKB:P06537, PubMed:25775514, PubMed:27120390, PubMed:9590696}.; [Isoform Alpha]: Has transcriptional activation and repression activity (PubMed:15866175, PubMed:19248771, PubMed:20484466, PubMed:23820903, PubMed:11435610, PubMed:15769988, PubMed:17635946, PubMed:19141540, PubMed:21664385). Mediates glucocorticoid-induced apoptosis (PubMed:23303127). Promotes accurate chromosome segregation during mitosis (PubMed:25847991). May act as a tumor suppressor (PubMed:25847991). May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity). {UniProtKB:P06537, PubMed:11435610, PubMed:15769988, PubMed:15866175, PubMed:17635946, PubMed:19141540, PubMed:19248771, PubMed:20484466, PubMed:21664385, PubMed:23303127, PubMed:23820903, PubMed:25847991}.; [Isoform Beta]: Acts as a dominant negative inhibitor of isoform Alpha (PubMed:7769088, PubMed:8621628, PubMed:20484466). Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed (PubMed:19248771, PubMed:26711253). Loses this transcription modulator function on its own (PubMed:20484466). Has no hormone-binding activity (PubMed:8621628). May play a role in controlling glucose metabolism by maintaining insulin sensitivity (By similarity). Reduces hepatic gluconeogenesis through down-regulation of PEPCK in an isoform Alpha-dependent manner (PubMed:26711253). Directly regulates STAT1 expression in isoform Alpha-independent manner (PubMed:26711253). {UniProtKB:P06537, PubMed:19248771, PubMed:20484466, PubMed:26711253, PubMed:7769088, PubMed:8621628}.; [Isoform Alpha-2]: Has lower transcriptional activation activity than isoform Alpha. Exerts a dominant negative effect on isoform Alpha trans-repression mechanism (PubMed:20484466).; [Isoform GR-P]: Increases activity of isoform Alpha. {PubMed:11358809}.; [Isoform Alpha-B]: More effective than isoform Alpha in transcriptional activation, but not repression activity. {PubMed:11435610, PubMed:15866175}.; [Isoform 10]: Has transcriptional activation activity. {PubMed:20484466}.; [Isoform Alpha-C1]: Has transcriptional activation activity. {PubMed:15866175}.; [Isoform Alpha-C2]: Has transcriptional activation activity. {PubMed:15866175}.; [Isoform Alpha-C3]: Has highest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127). Mediates glucocorticoid-induced apoptosis (PubMed:23303127, PubMed:23820903). {PubMed:15866175, PubMed:23303127, PubMed:23820903}.; [Isoform Alpha-D1]: Has transcriptional activation activity. {PubMed:15866175}.; [Isoform Alpha-D2]: Has transcriptional activation activity. {PubMed:15866175}.; [Isoform Alpha-D3]: Has lowest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127). {PubMed:15866175, PubMed:23303127, PubMed:23820903}.
Research Areas Hormones/Proteins
Handling
Storage This antibody is stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions This antibody has been used successfully in Western blot assays (1:500-1:1000 dilution) to identify human glucocorticoid receptor. Other applications are under investigation.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet