Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody (56575)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF Human, Mouse, Rat
SKU: 56575 Categories: ,
Overview
Product Name Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody (56575)
Description Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse Monoclonal Antibody
Target Glial Fibrillary Acidic Protein (GFAP)
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,ICC/IF
Host Mouse
Clonality Monoclonal
Clone ID S206B-9
Isotype IgG1
Immunogen Synthetic peptide corresponding to aa 411-422 (KTVEMRDGEVIK) of human GFAP. This sequence is 100
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.1% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat GFAP.
Target Name Glial fibrillary acidic protein
Target ID Glial Fibrillary Acidic Protein (GFAP)
Uniprot ID P14136
Alternative Names GFAP
Gene Name GFAP
Sequence Location Cytoplasm
Biological Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Research Areas Neuroscience
Background Glial fibrillary acidic protein (GFAP) is an intermediate filament protein that is expressed by numerous cell types of the central nervous system. It is involved in many important CNS processes, including cell communication and the functioning of the blood brain barrier. It is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell's cytoskeleton. GFAP is thought to help to maintain astrocyte strength and shape. There are multiple disorders associated with improper GFAP regulation. GFAP levels are used as a marker of neurologic damage in adults who suffer strokes and traumatic brain injuries.
Application Images
Description Western Blot analysis of Rat Brain Membrane showing detection of GFAP protein using Mouse Anti-GFAP Monoclonal Antibody, Clone S206B-9 (56575). Primary Antibody: Mouse Anti-GFAP Monoclonal Antibody (56575) at 1:250.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GFAP R416WT Monoclonal Antibody, Clone S206B-9 (56575). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GFAP R416WT Monoclonal Antibody (56575) at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GFAP R416WT Antibody (D) Composite.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GFAP R416WT Monoclonal Antibody, Clone S206B-9 (56575). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GFAP R416WT Monoclonal Antibody (56575) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GFAP R416WT Antibody. (D) Composite.
Additional Information
Handling
Storage This product is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-5ug/mL. A band of ~50kDa is detected.

Immunofluorescence: use at 10ug/mL.

These are recommended concentrations.

Endusers should determine optimal concentrations for their application.
References & Data Sheet