Anti-FLIP (CT) Antibody (2422)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF Human
SKU: 2422 Categories: , ,
Overview
Product Name Anti-FLIP (CT) Antibody (2422)
Description Anti-FLIP (CT) Rabbit Polyclonal Antibody
Target FLIP (CT)
Species Reactivity Human
Applications ELISA,WB,ICC,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 447- 464 at the C-terminus of human FLIPa/FLIPL (accession no. AAC51622)
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes full-length human, mouse, and rat FLIPalpha (55kDa).
Target Name CASP8 and FADD-like apoptosis regulator
Target ID FLIP (CT)
Uniprot ID O15519
Alternative Names Caspase homolog, CASH, Caspase-eight-related protein, Casper, Caspase-like apoptosis regulatory protein, CLARP, Cellular FLICE-like inhibitory protein, c-FLIP, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, I-FLICE, MACH-related inducer of toxicity, MRIT, Usurpin [Cleaved into: CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12]
Gene Name CFLAR
Gene ID 8837
Accession Number NP_001120655.1
Biological Function Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. {PubMed:9880531}.
Research Areas Apoptosis
Background Caspase-8 (FLICE) and -10 (FLICE2) are two pivotal members of the ICE/CED-3 protease family. FLICE-inhibitory proteins have been identified in viruses and human cells and are designated v-FLIPs and FLIP, respectively. Human FLIP was cloned by several independent laboratories and designated Casper, I-FLICE, FLAME-1, CASH, and CLARP. FLIP contains two death effector domains and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and -10 and potently inhibits apoptosis induced by death receptors CD95, DR3, TRAIL-R, and TNFR1. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, g, and delta, respectively.
Application Images
Description Western blot analysis of FLIP in HeLa (H), Jurkat (J), and K562 (K) whole cell lysate with FLIP antibody at 1:1000 dilution.
Description Immunocytochemistry of FLIP in Jurkat cells with FLIP antibody at 10 ug/mL.
Description Immunofluorescence of FLIP in Jurkat cells with FLIP antibody at 10 ug/mL.
Description Immunofluorescence of FLIP in K562 cells with FLIP antibody at 5 ug/mL. Red: FLIP Antibody (2422)
Blue: DAPI staining
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Immunocytochemistry: use at 10ug/mL.

Positive control: Whole cell lysate from HeLa cells.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
References & Data Sheet