Anti-Fibronectin Antibody (42040)

$497.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 400ug WB,ELISA,Inhibition,IHC Human, Bovine
SKU: 42040 Categories: , ,
Overview
Product Name Anti-Fibronectin Antibody (42040)
Description Anti-Human Fibronectin Mouse Monoclonal Antibody
Target Fibronectin
Species Reactivity Human, Bovine
Applications WB,ELISA,Inhibition,IHC
Host Mouse
Clonality Monoclonal
Clone ID FN12-8
Isotype IgG1
Immunogen Human plasma fibronectin
Properties
Form Lyophilized
Formulation PBS, pH 7.4, 1% BSA, lyophilized.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Protein Stabilizer 1% Bovine Serum Albumin
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity This antibody recognizes an epitope in the cell-binding domain of human and bovine fibronectin and can inhibit cell adhesion.
Target Name Fibronectin
Target ID Fibronectin
Uniprot ID P02751
Alternative Names FN, Cold-insoluble globulin, CIG [Cleaved into: Anastellin; Ugl-Y1; Ugl-Y2; Ugl-Y3]
Gene Name FN1
Sequence Location Secreted, extracellular space, extracellular matrix
Biological Function Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617). {UniProtKB:P11276, PubMed:3024962, PubMed:34089617, PubMed:3593230, PubMed:3900070, PubMed:7989369}.; [Anastellin]: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. {PubMed:11209058, PubMed:15665290, PubMed:19379667, PubMed:8114919}.
Research Areas Cell adhesion
Application Images
Description Immunohistochemistry: 1-10ug/ml, paraffin-embedded or frozen tissue sections with Proteinase K treatment for antigen retrieval. Staining of fibronectin (FN) in human well-differentiated colon cancer tissue (bottom) and moderatelydifferentiated colon cancer (top) with #42040.
Additional Information
Handling
Storage The stock solution (2. 0mg/ml) can be stored in aliquots at -20°C for 1 year or can be stored at 4°C for 6 months after adding 0.1% sodium azide. Prepare dilutions of stock solution immediately before use. Do not store diluted stock solution. Avoid repeated freeze-thaw cycles
Dilution Instructions Reconstitute lyophilized antibody in 200ul of distilled water (final concentration 2.0mg/ml).
Application Instructions ELISA: 1-10ug/ml with immobilized antigen.Western Blot: 1-10ug/ml, reducing or non-reducing conditions.Cell Adhesion Inhibition Assay: 0.1-1.0ug/ml on 10ug/ml fibronectin-coated microwells inhibits adhesion of trypsinized NRK cells.

Immunohistochemistry: 1-10ug/ml, paraffin-embedded or frozen tissue sections with Proteinase K treatment for antigen retrieval.Staining of fibronectin (FN) in human well-differentiated colon cancer tissue (bottom) and moderately- differentiated colon cancer (top) with #42040.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet