Anti-ECS Antibody (18830-01)

$426.00

Host Quantity Applications Species Reactivity Data Sheet  
Goat 100ug WB,IP,ICC,ELISA Ecs
SKU: 18830-01 Categories: , ,
Overview
Product Name Anti-ECS Antibody (18830-01)
Description Anti-ECS Goat Polyclonal Antibody
Target ECS
Species Reactivity Ecs
Applications WB,IP,ICC,ELISA
Host Goat
Clonality Polyclonal
Immunogen DDDDK conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.2, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.09% Sodium Azide
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity Antibody was affinity-purified on the peptide.
Target ID ECS
Uniprot ID P98073
Gene ID 5651
CAS Number 9014-74-8
Sequence Location Membrane, Single-pass type II membrane protein
Biological Function Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
Research Areas Epitope Tags
Background Enterokinase, also known as enteropeptidase, is a serine protease that catalyzes the hydrolysis of peptide bonds in proteins but, unlike other kinases, does not catalyze transfer of phosphate groups. Enterokinase cleaves proteins following a lysine at an enterokinase cleavage site, or ECS (Asp-Asp-Asp-Asp-Lys). In vivo, this cleavage results in trypsin-dependent activation of other pancreatic zymogens, such as chymotrypsinogen, proelastase, procarboxypeptidase and prolipase in the lumen of the gut.
Application Images
Description Detection of ECS (DYKDDDDK)-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein.Antibody: Affinity purified goat anti-ECS (DYKDDDDK) antibody 18830 used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at 2-8°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000- 1:20,000.

Immunocytochemistry: use at a dilution of 1:100-1:400.

ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500.

Immunoprecipitation: use 1-4ug/mg lysate

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications. Detection of ECS-tagged protein in 200, 100, and 50ng of E. coli lysate containing tagged fusion protein with #18830 diluted 1:25,000.
References & Data Sheet