Anti-E2F-1 Antibody (3333)

$503.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IP Human
SKU: 3333 Categories: , ,
Overview
Product Name Anti-E2F-1 Antibody (3333)
Description Anti-E2F-1 Mouse Monoclonal Antibody
Target E2F-1
Species Reactivity Human
Applications WB,IP
Host Mouse
Clonality Monoclonal
Clone ID 6B5
Isotype IgG1
Immunogen GST fusion protein expressed in E. coli corresponding to aa 162-476 of human E2F-1.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes an epitope within aa 223-322 of human E2F-1.
Target Name Transcription factor E2F1
Target ID E2F-1
Uniprot ID Q01094
Alternative Names E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1
Gene Name E2F1
Gene ID 1869
Accession Number NP_005216
Sequence Location Nucleus
Biological Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 rPubMed:10675335, PubMed:12717439, PubMed:17050006, PubMed:17704056, PubMed:18625225, PubMed:20040599, PubMed:20176812, PubMed:28992046, PubMed:8170954}.
Research Areas Cancer Research
Application Images
Description Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [6B5] (3333) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
Description Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [6B5] (3333) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-5 ug/mL.

Immunoprecipitation: use at 1-5 ug/mL.

Positive controls: E2F-1 transfected Soas2 cells and recombinant fusion protein.
References & Data Sheet