Anti-DRAK2 (CT) Antibody (2149)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC Human
SKU: 2149 Categories: , ,
Overview
Product Name Anti-DRAK2 (CT) Antibody (2149)
Description Anti-DRAK2 (CT) Rabbit Polyclonal Antibody
Target DRAK2 (CT)
Species Reactivity Human
Applications ELISA,WB,ICC
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 351-365 of human DRAK2 (accession no. AB011421).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human DRAK2 (45kDa). No cross-reactivity with DRAK1, DAP or ZIP kinases.
Target Name Serine/threonine-protein kinase 17B
Target ID DRAK2 (CT)
Uniprot ID O94768
Alternative Names EC 2.7.11.1, DAP kinase-related apoptosis-inducing protein kinase 2
Gene Name STK17B
Gene ID 9262
Accession Number NP_004217.1
Sequence Location Nucleus, Cell membrane, Endoplasmic reticulum-Golgi intermediate compartment
Biological Function Phosphorylates myosin light chains (By similarity). Acts as a positive regulator of apoptosis. {PubMed:9786912}.
Research Areas Apoptosis
Background Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis have been identified and designated DRAK1 and DRAK2 for DAP kinase–related apoptosis-inducing protein kinases. DRAKs contain an N-terminal kinase domain and a C- terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases which mediate apoptosis through their catalytic activities. DRAK2 is located in cell nuclei, and the mRNA for DRAK2 is ubiquitously expessed in human tissues.
Application Images
Description Western blot analysis of DRAK2 in Raji cell lysate with DRAK2 antibody at (A) 1 and B (2) ug/mL.
Description Immunocytochemistry of DRAK2 in Jurkat cells with DRAK2 antibody at 10 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Immunocytochemistry: use at 10ug/mL.

Positive control: Whole cell lysate from Jurkat or Raji cells.

These are recommended concentrations.

Enduser should determine optimal concentrations or their applications.
References & Data Sheet