Anti-DR3 (ED) Antibody (2401)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF Human
SKU: 2401 Categories: , ,
Overview
Product Name Anti-DR3 (ED) Antibody (2401)
Description Anti-DR3 (ED) Rabbit Polyclonal Antibody
Target DR3 (ED)
Species Reactivity Human
Applications ELISA,WB,ICC,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to the extracellular domain of human DR3 precursor (accession no. AAQ88676).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes full-length human and mouse DR3 (59kDa).
Target Name Tumor necrosis factor receptor superfamily member 25
Target ID DR3 (ED)
Uniprot ID Q93038
Alternative Names Apo-3, Apoptosis-inducing receptor AIR, Apoptosis-mediating receptor DR3, Apoptosis-mediating receptor TRAMP, Death receptor 3, Lymphocyte-associated receptor of death, LARD, Protein WSL, Protein WSL-1
Gene Name TNFRSF25
Gene ID 8718
Accession Number NP_683868
Sequence Location [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; [Isoform 9]: Cell membrane; Single-pass type I membrane protein.; [Isoform 11]: Cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted.; [Isoform 4]: Secreted.; [Isoform 5]: Secreted.; [Isoform 6]: Secreted.; [Isoform 7]: Secreted.; [Isoform 8]: Secreted.; [Isoform 10]: Secreted.; [Isoform 12]: Secreted.
Biological Function Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis. {PubMed:8875942, PubMed:8994832, PubMed:9052839}.
Research Areas Apoptosis
Background Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain-containing receptors, TNFR1 and Fas. Another cell death receptor was recently identified and designated DR3, Wsl-1, Apo-3, TRAMP, and LARD. DR3 is widely expressed in tissues enriched in lymphocytes including peripheral blood, thymus, and spleen. Like TNFR1, DR3 induces apoptosis and NF- B activation.
Application Images
Description Western blot analysis of DR3 in Jurkat total cell lysate with DR3 antibody at 1:1000 dilution.
Description Immunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 ug/mL.
Description Immunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 ug/mL.
Description Immunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 ug/mL. Green: DR3 Antibody (2401)
Blue: DAPI staining
Description Immunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Immunocytochemistry: use at 10ug/mL.

Positive control: Whole cell lysate from Jurkat cells.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
References & Data Sheet