Anti-DNase II (CT) Antibody (2059)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Human
SKU: 2059 Categories: , ,
Overview
Product Name Anti-DNase II (CT) Antibody (2059)
Description Anti-Dnase II (CT) Rabbit Polyclonal Antibody
Target DNase II (CT)
Species Reactivity Human
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 347- 360 of human DNase II precursor (accession no. AF047016).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes the proenzyme of human DNase II (40kDa).
Target Name Deoxyribonuclease-2-α
Target ID DNase II (CT)
Uniprot ID O00115
Alternative Names EC 3.1.22.1, Acid DNase, Deoxyribonuclease IIα, DNase IIα, Lysosomal DNase II, R31240_2
Gene Name DNASE2
Gene ID 1777
Accession Number NP_001366.1
Sequence Location Lysosome.
Biological Function Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.
Research Areas Apoptosis
Background DNase II causes both chromatin condensation and DNA fragmentation. The genes encoding human, porcine, and murine DNase II have been cloned. The DNase II gene encodes a 40kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32kDa ( ) and12 kDa ( ) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation. DNase II is ubiquitously expressed in human tissues.
Application Images
Description Western blot analysis of DNase II in human spleen tissue lysate in the absence (lane 1) or presence (lane 2) of blocking peptide with DNase antibody II at 1:500 dilution.
Description Immunohistochemistry of DNaseII in human spleen tissue with DNaseII antibody at 5 ug/mL.
Description Immunofluorescence of DNase II in human spleen tissue with DNase II antibody at 5 ug/ml. Green: DNase II antibody (2059)
Red: Phylloidin staining
Blue: DAPI staining
Description Immunohistochemistry of DNase II in human spleen tissue with DNase II antibody at 5 ug/ml.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Immunohistochemistry: use at 5ugml.

Positive control: Tissue lysate of human spleen.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
References & Data Sheet