Anti-DMPO Nitrone Adduct Antibody (12531)

$479.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF,IP,ELISA,AM All
SKU: 12531 Categories: , ,
Overview
Product Name Anti-DMPO Nitrone Adduct Antibody (12531)
Description Anti-DMPO Nitrone Adduct Mouse Monoclonal Antibody
Target DMPO Nitrone Adduct
Species Reactivity All
Applications WB,IHC,ICC/IF,IP,ELISA,AM
Host Mouse
Clonality Monoclonal
Clone ID N1664A
Isotype IgG1
Immunogen 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to ovalbumin.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts, and DMPO-DNA adducts. It does not cross-react with non-adducted proteins or DNA. Species independent.
Target ID DMPO Nitrone Adduct
Research Areas DNA
Background Free radicals are highly reactive and generally short-lived species. Hence, most studies of free radicals have been restricted to analysis of end products formed following their interaction with cellular biomolecules. One technology that has permitted direct study of free radicals is electron spin resonance (ESR). For very short lived radicals, spin traps have been employed to generate longer lived radicals that are more amenable to ESR analysis. The most widely used of these spin traps is 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). The reaction of DMPO with a protein radical gives rise to a nitroxide radical which can be disproportionate to hydrozylamine and nitrone adducts. Of these species, only the nitrone adduct can be considered stable.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DMPO Monoclonal Antibody, Clone N1664A (12531). Tissue: macrophage cell line (Raw 264.7). Species: Mouse. Primary Antibody: Mouse Anti-DMPO Monoclonal Antibody (12531) at 1:100. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green) at 1:1000. Counterstain: DAPI (blue) nuclear stain.
Description Western Blot analysis of Human HL 60 clone 15 eosinophils lysates showing detection of DMPO protein using Mouse Anti-DMPO Monoclonal Antibody, Clone N1664A (12531). Primary Antibody: Mouse Anti-DMPO Monoclonal Antibody (12531) at 1:200.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-10ug/mL.

Immunofluorescence: use at10ug/mL.

These are recommended concentrations;

Enduser should determine optimal concentrations for their applications

ELISA: use at 1-10ug/mL

Positive control: DMPO nitrone adducts of metmyoglobin.
References & Data Sheet