Anti-CIKS/Act1 (NT) Antibody (56113)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Human
SKU: 56113 Categories: , ,
Overview
Product Name Anti-CIKS/Act1 (NT) Antibody (56113)
Description Anti-CIKS/Act1 (NT) Rabbit Polyclonal Antibody
Target CIKS/Act1 (NT)
Species Reactivity Human
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Immunogen Peptide corresponding to aa 2-15 of human CIKS.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes an epitope at the N-terminus of human CIKS/Act1 (Connection to IKK and SAPK/JNK), an activator of IKK. CIKS/Act1 interacts directly with IKKg and also activates activating transcription factor (ATF) and activator protein 1 (AP-1) through Jun kinase (JNK). CIKS/Act1 is associated with inflammation and stress responses and is expressed in a variety of human tissues.
Target Name E3 ubiquitin ligase TRAF3IP2
Target ID CIKS/Act1 (NT)
Uniprot ID O43734
Alternative Names EC 2.3.2.27, Adapter protein CIKS, Connection to IKK and SAPK/JNK, E3 ubiquitin-protein ligase CIKS, Nuclear factor NF-κ-B activator 1, ACT1, TRAF3-interacting protein 2
Gene Name TRAF3IP2
Biological Function E3 ubiquitin ligase that catalyzes 'Lys-63'-linked polyubiquitination of target protein, enhancing protein-protein interaction and cell signaling (PubMed:19825828). Transfers ubiquitin from E2 ubiquitin-conjugating enzyme UBE2V1-UBE2N to substrate protein (PubMed:19825828). Essential adapter molecule in IL17A-mediated signaling (PubMed:19825828, PubMed:24120361). Upon IL17A stimulation, interacts with IL17RA and IL17RC receptor chains through SEFIR domains and catalyzes 'Lys-63'-linked polyubiquitination of TRAF6, leading to TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways (PubMed:19825828). {PubMed:19825828, PubMed:24120361, PubMed:33723527}.
Research Areas Neuroscience
Application Images
Description Western blot analysis of CIKS expression in human lung (lane A) and placenta (lane B) tissue lysates with CIKS antibody at 1 ug/ml.
Description Immunohistochemistry of CIKS in human lung tissue with CIKS antibody at 5 ug/mL.
Description Immunofluorescence of CIKS in human lung tissue with CIKS antibody at 20 ug/mL.
Description Immunofluorescence of CIKS in A549 cells with CIKS antibody at 20 ug/mL. Green: CIKS Antibody (56113)
Blue: DAPI staining
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-1 ug/mL. In immunoblots, a band of 63 kD is detected.

Positive control: Human placenta tissue lysate.
References & Data Sheet