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Anti-CD73/5’Nucleotidase Antibody (90006)

$520.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ELISA Human
SKU: 90006 Categories: , ,
Overview
Product Name Anti-CD73/5'Nucleotidase Antibody (90006)
Description Anti-CD73/5'-Nucleotidase Mouse Monoclonal Antibody
Target CD73/5'Nucleotidase
Species Reactivity Human
Applications WB,IHC,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 10f1
Isotype IgG1
Immunogen Recombinant 5'-nucleotidase (5'-NT). Accession no. P21589.
Properties
Form Lyophilized
Formulation Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
Buffer Formulation Tris Buffer
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody reacts with human 5'-NT.
Target Name 5'-nucleotidase
Target ID CD73/5'Nucleotidase
Uniprot ID P21589
Alternative Names 5'-NT, EC 3.1.3.5, Ecto-5'-nucleotidase, CD antigen CD73
Gene Name NT5E
Sequence Location Cell membrane; Lipid-anchor, GPI-anchor.
Biological Function Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. {PubMed:21933152}.
Research Areas Enzymes
Background 5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. CD73 converts AMP to adenosine. This enzyme consists of a dimer of 2 identical 70-kDa subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. A deficiency of NT5 occurs in a variety of immunodeficiency diseases
Application Images
Additional Information
Handling
Storage This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
Dilution Instructions Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.
Application Instructions ELISA: use at 1-10ug/ml

Immunoblotting:

User should use at 1-5ug/ml. A band of ~63kDa is detected.

Immunohistochemistry: use at 1-5ug/ml.

These are recommended concentrations.

End user should determine optimal concentrations for their applications.
References & Data Sheet