Anti-Cavβ2 Ca+2 Channel Antibody (11524)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF,IP,AM Human, Mouse, Rat
SKU: 11524 Categories: , ,
Overview
Product Name Anti-Cavβ2 Ca+2 Channel Antibody (11524)
Description Anti-Cavß Ca+2 Channel Mouse Monoclonal Antibody
Target Cavβ2 Ca+2 Channel
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,ICC/IF,IP,AM
Host Mouse
Clonality Monoclonal
Clone ID S8b-1
Isotype IgG1
Immunogen Synthetic peptide corresponding to aa 189-205 of rat Cavb2 (RSPKPSANSVTSPHSKE) (accession no. NP_446303).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat Cavb2. It does not cross-react with Cav b1, Cav b3, or Cav b4.
Target Name Voltage-dependent L-type calcium channel subunit β-2
Target ID Cavβ2 Ca+2 Channel
Uniprot ID Q8VGC3
Alternative Names CAB2, Calcium channel voltage-dependent subunit β 2
Gene Name Cacnb2
Accession Number NP_446303
Sequence Location Cell membrane, sarcolemma, Peripheral membrane protein, Cytoplasmic side
Biological Function The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting. {PubMed:11604404, PubMed:12042350, PubMed:1370480}.
Research Areas Ion Channels
Background Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Cavbeta subunits are involved in the transport of the pore-forming alpha1 subunit to the plasma membrane. They also shield an ER Retention signal on the alpha1 subunit, thereby guiding the pore- forming subunit to the target membrane.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav beta 2 Monoclonal Antibody, Clone S8B-1 (11524). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav beta 2 Monoclonal Antibody (11524) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav beta 2 Antibody (D) Composite.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (11524). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (11524) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive. Bright dottiness located throughout cytoplasm and in nuclei.
Description Immunohistochemistry analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (11524). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (11524) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All nuclei. Some nuclei also staining higher up in epidermis.
Description Western Blot analysis of Human Cell line lysates showing detection of Cav Beta2 Calcium Channel protein using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (11524). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (11524) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-10ug/mL. A band of ~78kDa is detected.

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: Rat brain lysate
References & Data Sheet